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Estrutura de Protenas

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Figure 2.5. O pI o pH onde a carga lquida da protena ZERO. A pHs menoresa protena tem carater CATINICO e a pHs acima do pI aninico.

Lembra que pKa invarivel o termo varivel o pH.

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Fora Inica e Enovelamento de Protenas.

AAs bsico e cidos

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-S-S-, H///O, cargas, VDW, uria

A partir da seq. 1ria, possibilidades de enovelamento enorme!!

Como sabe?? Protenas que ajudam no Enovelamento Folding

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Efeito de ons,

Espcificidade de ons, Caotrpicos e CosmotrpicosDenaturam Naturam

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1 = 1,5 Anstieg pro Aminosurerest2 = 5,4 = 3,6 Aminosurereste proWindung3 = 27 entspricht 18 Aminosurereste

auf 5 Windun en4 = 26

5 = 5,1 w1 - w5 = je 1 Helix-windung

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3,6 resduos de aminocido por passo ~ 5.4 Estabilizado por H-ponte entre.Como devem ser os grupos R? e o interior da

Hlice??

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.

Folha anti paralela

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endothelin angiogenin lysozyme mellitin

collagen thioredoxin hemoglobin

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Brookhaven Protein Data Bank

The Brookhaven Data Bank will contain the most recent entries of all protein structural

data. Proteins can easily be searched by using Brookhaven's PDB Gopher. When thebox appears type in any key word. If a match appears, select the protein of your choice.Three files will appear: *.biblio, *.full, and *.gif. The first file will only give theinformation found in the header, it will not give coordinate data, the *.full file gives alldata and is needed if you wish to manipulate structures from your own terminal. The

*.gif file will contain a single image of the molecule. Remember that many casesseveral structures will be found for the same keyword. As an example try typing inpepsin after clicking here Next click on one of the many structures that show up on

* * *. . , . , .

Brookhaven.For more information about the Brookhaven Protein Data Base connectto their home directory which contains FTP access to recent PDBNewsletter. A searchable index by key words can be used to search the

Newsletter.

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Protenas de membrana:

-Integrais-Perifricas

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Figure 4.19

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Name Type Specificity

CyanogenBromide

Chemical Carboxyl Side of Methionine

Trypsin Enzymatic Carboxyl Side of Basic AminoAcidse.g. Lys & Arg

Chymotrypsin Enzymatic Carboxyl Side of Aryl Amino

Acidse.g. Phe, Tyr & Trp

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Sntese de Peptdeos

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The Merrifield Peptide Synthesis

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The Merrifield Peptide Synthesis

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