ecoriver seminÁrio final

ECORIVERSEMINRIO FINALAVALIAO QUMICA E ECOTOXICOLGICA DAS GUAS RESIDUAIS

Seminrio ECORIVER ia, 29 de Setembro de 2005, Ana Morbey

Caracterizao ecotoxicolgica das guas residuais Proposta de ferramenta para avaliao da toxicidade Curso de formao na rea da Ecotoxicologia



Qumica 2 campanhas 2003 / 2 campanhas 2004

Parmetro

SectorCBOCQOSSTpHCNFenoisFluorHidNitSulfO&GmetaisAlimentar Grfico Oficinas Qumico Trat sup Outros ETAR


PARMETROS QUMICOSClassificao de acordo com:Regulamento dos Servios Municipalizados de Loures(indstrias que descarregam no Sistema Municipal)

- Decreto-Lei n 236, de 1 de Agosto de 1998 (indstrias que descarregam directamente no Rio Tranco)

-Tabela do Instituto da gua para Classificao daQualidade das guas Superficiais para Fins Mltiplos


CLASSIFICAO QUMICA GLOBAL

= INDSTRIAS =


CLASSIFICAO QUMICA GLOBAL= ETAR =

indstrias

Aos valores x foi adicionada metade do dito valor (limite mximo de quantificao)Sector Qumicopontualcomposta

CDIGOC14C15C44F2F30F4F7O18O39O8P17P37R24R27ST41CDIGOC14C15C44F2F30F4F7O18O39O8P17P37R24R27ST41C14C14C14C14C15C15C15C15C44C44C44C44C44

EMPRESASEBOLLECIFARMAHOVIONEKILOMALCNTARAENBCOPAMMECFAPAJALSOCORSULLITHOFORMASGRAF& LITOSALV CAETANHENRIQ. GOMES FPOLICROMOEMPRESASEBOLLECIFARMAHOVIONEKILOMALCNTARAENBCOPAMMECFAPAJALSOCORSULLITHOFORMASGRAF& LITOSALV CAETANHENRIQ. GOMES FPOLICROMOSEBOLLECIFARMAHOVIONE

COLHEITAMaio20/5/0322/5/0329/5/0320/5/0328/5/0322/5/0328/5/0328/5/0326/5/0326/5/0327/5/0327/5/0327/5/0328/5/0329/5/03COLHEITAMay-0320/5/0322/5/0329/5/0320/5/0328/5/0322/5/0328/5/0328/5/0326/5/0326/5/0327/5/0327/5/0327/5/0328/5/0329/5/03May-03Nov-03Apr-04Sep-04May-03Nov-03Apr-04Sep-04May-03Nov-03Apr-04May-04Sep-04

CBO5mg/l38.39391170-217064.25835.855.42500118023438.420034.8CBO5mg/l38.39391170-217064.25835.855.42500118023438.420034.8mg/lCBO538.311226020093957392512301170315019562633436

CQOmg/l24027022288100614441034844177108119203211041192560323CQOmg/l24027022288100614441034844177108119203211041192560323CQO24016054535827021240182729702288415845184777891

SSTmg/l152314020016931938419409455014814111309428SSTmg/l152314020016931938419409455014814111309428SST15715230023172026140400200200058

Cianetosug/l< 10

Critrios de seleco

ECOTOXICIDADEPontosCE50 > 10% ...............................0CE50 < 10% ...............................1

BIODEGRADABILIDADECBO5 / CQO > 40%..................0CBO5 / CQO < 40%..................1

DESCARGA NO RION ............................................ ..0S............................................... 1


Os ensaios medem efeitos em indivduos de uma dada espcie, sejam efeitos de letalidade ou imobilizao, de inibio da reproduo ou do crescimento.

Na avaliao da ecotoxicidade --- pelo menos duas ou trs espcies e ter presente o tipo de ecossistema objecto da descarga final. BIOENSAIOS


Daphnia magnaVibrio fischeriLemna minorPseudokirchneriella subcapitataThamnocephalus platyurusOs ensaios medem efeitos em indivduos de uma dada espcie, sejam efeitos de letalidade ou imobilizao, de inibio da reproduo ou do crescimento.



UTILIZAO DE SOLUES DE REFERNCIA Ex: Fenol, Dicromato de potssioREPLICADOS DAS AMOSTRASENSAIOS DE INTERCALIBRAOCOMO GARANTIR A QUALIDADE DOS RESULTADOS?ACREDITAO DE MTODOS


Intercalibrao

Amostras reais

Boa correlao entre os resultados obtidos para os ensaios realizados entre parceiros

Exerccios interlaboratoriais

Bom desempenho dos parceiros em exerccios de intercalibrao internacionais


Anlise componentes principais

2 primeiras componentes principais, P1 e P2, justificam 77,6% das variveis.

P1 associada toxicidade para os crustceos (50,2%)

P2 associada toxicidade para a bactria (27,4%)


Grfico PCA

2.12240724-1.0570601-1.0570601-1.0570601-1.0570601-1.0570601-1.0570601-0.1369412

-0.27158220.677753280.677753280.677753280.677753280.677753280.67775328-0.0949313

0.50727667-2.3799126-2.3799126-2.3799126-2.3799126-2.3799126-2.37991260.58898703

-2.0956563-0.3642718-2.0956563-2.0956563-2.0956563-2.0956563-2.09565630.56843167

-0.7378951-0.7878172-0.7378951-0.7378951-0.7378951-0.7378951-0.7378951-0.0437222

-1.750534-0.7692754-1.750534-1.750534-1.750534-1.750534-1.750534-0.35711

0.46499443-1.00470650.464994430.464994430.464994430.464994430.46499443-0.415663

-1.4424152-1.4424152-1.2505643-1.4424152-1.4424152-1.4424152-1.4424152

-2.6142364-2.6142364-0.1899039-2.6142364-2.6142364-2.6142364-2.6142364

2.396894232.39689423-0.65960422.396894232.396894232.396894232.39689423

3.247945613.247945611.004109993.247945613.247945613.247945613.24794561

1.038717291.038717290.152110431.038717291.038717291.038717291.03871729

2.232979482.232979482.232979481.145499832.232979482.232979482.23297948

-2.4496322-2.4496322-2.4496322-0.7119235-2.4496322-2.4496322-2.4496322

3.247945613.247945613.247945613.247945611.004109993.247945613.24794561

-1.6342661-1.6342661-1.6342661-1.6342661-0.4828898-1.6342661-1.6342661

-2.1389764-2.1389764-2.1389764-2.1389764-2.13897640.01867212-2.1389764

3.37187972-1.5272736-1.5272736-1.5272736-1.5272736-1.5272736-1.5272736

0.404566152.77287832.77287832.77287832.77287832.77287832.7728783

2.3658554-2.4115647-2.4115647-2.4115647-2.4115647-2.4115647-2.4115647

-1.5904777-0.6040563-1.5904777-1.5904777-1.5904777-1.5904777-1.5904777

-0.89324672.10662497-0.8932467-0.8932467-0.8932467-0.8932467-0.8932467

-0.0942438-1.0521696-0.0942438-0.0942438-0.0942438-0.0942438-0.0942438

1.76294982-1.56755311.762949821.762949821.762949821.762949821.76294982

0.453920.45392-1.70721450.453920.453920.453920.45392

-2.2399235-2.23992353.14287588-2.2399235-2.2399235-2.2399235-2.2399235

1.858102051.85810205-0.54781111.858102051.858102051.858102051.85810205

1.754733231.75473323-0.22780711.754733231.754733231.754733231.75473323

1.267935641.26793564-1.51398561.267935641.267935641.267935641.26793564

-0.5154375-0.5154375-0.5154375-1.1670631-0.5154375-0.5154375-0.5154375

1.060873311.060873311.06087331-1.60066821.060873311.060873311.06087331

2.563919382.563919382.563919382.563919382.04223192.563919382.56391938

-0.6061509-0.6061509-0.6061509-0.6061509-1.262203-0.6061509-0.6061509

-3.0984203-3.0984203-3.0984203-3.0984203-3.09842031.16103527-3.0984203

0.743235020.743235020.743235020.743235020.743235020.74323502-1.6166055

-0.7315845-0.7315845-0.7315845-0.7315845-0.7315845-0.7315845-0.9132592

-0.6924507-0.6924507-0.6924507-0.6924507-0.6924507-0.6924507-0.3229265

-0.7512987-0.7512987-0.7512987-0.7512987-0.7512987-0.7512987-1.2134847

3.152941973.152941973.152941973.152941973.152941973.152941971.14829359

-1.0705184-1.0705184-1.0705184-1.0705184-1.0705184-1.07051840.46992615

-0.8716019-0.8716019-0.8716019-0.8716019-0.8716019-0.8716019-0.8000332

3.26839967-2.3645-2.3645-2.3645-2.3645-2.3645-2.3645

0.47384330.274863930.274863930.274863930.274863930.274863930.27486393

1.0314788-2.3726808-2.3726808-2.3726808-2.3726808-2.3726808-2.3726808

-3.30293440.58591128-3.3029344-3.3029344-3.3029344-3.3029344-3.3029344

-1.16312873.45768892-1.1631287-1.1631287-1.1631287-1.1631287-1.1631287

-1.2699173-0.7969928-1.2699173-1.2699173-1.2699173-1.2699173-1.2699173

-0.1377268-0.1730437-0.1377268-0.1377268-0.1377268-0.1377268-0.1377268

0.327084250.32708425-1.02429490.327084250.327084250.327084250.32708425

-1.0316007-1.0316007-0.7994097-1.0316007-1.0316007-1.0316007-1.0316007

3.155792083.155792081.143968083.155792083.155792083.155792083.15579208

3.247945613.247945611.004109993.247945613.247945613.247945613.24794561

-0.6502958-0.6502958-1.5063163-0.6502958-0.6502958-0.6502958-0.6502958

1.903627171.903627171.903627170.633721981.903627171.903627171.90362717

-2.5414438-2.5414438-2.5414438-0.4207296-2.5414438-2.5414438-2.5414438

2.732842682.732842682.732842682.732842681.663005062.732842682.73284268

-2.7977832-2.7977832-2.7977832-2.79778320.17412317-2.7977832-2.7977832

2.265971422.265971422.265971422.265971422.265971422.051120362.26597142

0.829698620.829698620.829698620.829698620.829698620.82969862-1.3918476

2.322029592.322029592.322029592.322029592.322029592.322029591.28748348

-0.2792253-0.2792253-0.2792253-0.2792253-0.2792253-0.2792253-1.0456071

0.168249980.168249980.168249980.168249980.168249980.16824998-1.1760092

-0.0676924-0.0676924-0.0676924-0.0676924-0.0676924-0.0676924-1.4675081

3.247945613.247945613.247945613.247945613.247945613.247945611.00410999

2.030946262.030946262.030946262.030946262.030946262.03094626-0.916687

-0.5069768-0.5069768-0.5069768-0.5069768-0.5069768-0.5069768-0.7297348

1.294389471.294389471.294389471.294389471.294389471.29438947-1.7085396

2.226406982.226406982.226406982.226406982.226406982.226406981.64365034

-0.4034972-0.4034972-0.4034972-0.4034972-0.4034972-0.4034972-1.5216597

-0.3883165-0.3883165-0.3883165-0.3883165-0.3883165-0.3883165-1.5649823

1.011446291.011446291.011446291.011446291.011446291.01144629-1.7779813

-2.8645944-2.8645944-2.8645944-2.8645944-2.8645944-2.86459440.56704517

-0.2008673-0.2008673-0.2008673-0.2008673-0.2008673-0.2008673-1.5264117

Vf=15 min

Tp=24 h

DM Kit

DM

Ps Kit

L minor

+ Ps+ Lemna- Vibrio- crustceos

+ Ps+ Lemna- Vibrio+ crustceos

- Ps- Lemna+ Vibrio+ crustceos

- Ps- Lemna+ Vibrio- crustceos

Qumico

Alimentar

Outro

Grfico

Oficinas

Trat. sup.

ETAR

Correlao

PC1

PC2

PCA - Dados Ecotoxicolgicos

Dados para Grfico PCA

DMDM KitVf-5minVf-15minTp-24hPs kitL minorPC1PC2PC2PC2PC2PC2PC2PC2

262.39.4710074.57.8610043.5-1.05706012.12240724

2710024.252.146.540.61001000.67775328-0.2715822

2814.715.915.712.31268.817.5-2.37991260.50727667

291.322.56.814.7216.664.965-2.0956563-0.3642718

3060.931.811.19.5533.310062.8-0.7378951-0.7878172

313.19.497.367.9324.610075-1.750534-0.7692754

3216.510019.924.455.51001000.46499443-1.0047065

3315.45.64.75.0226.1100100-1.4424152-1.2505643

3435.68.15.621.123.08100-2.6142364-0.1899039

3510010044.4461001001002.39689423-0.6596042

361001001001001001001003.247945611.00410999

3781.810040.64070.3101001.038717290.15211043

3810063.610010059.41001002.232979481.14549983

3915.60.130.273.9237100-2.4496322-0.7119235

401001001001001001001003.247945611.00410999

4117.531.896.6917.410048.6-1.6342661-0.4828898

4214.88.222.414.35.6212100-2.13897640.01867212

5113.114.810090.35.6239.810-1.52727363.37187972

5210010074.776.3100100902.77287830.40456615

5310.618.954.449.25.6212.86.3-2.41156472.3658554

542431.91.741.0730.768.961.3-1.5904777-0.6040563

5541.430.765.971.927.741.429.8-0.89324672.10662497

5635.164.815.713.947.510090-0.0942438-1.0521696

5710010010.111100100901.76294982-1.5675531

5810061.81.020.5750.5100900.45392-1.7072145

590.82.8710060.31.5729.76.3-2.23992353.14287588

6010010037.527.1100100651.85810205-0.5478111

6110010043.127.710010049.31.75473323-0.2278071

6210063.68.3210.2100100901.26793564-1.5139856

6359.245.84.133.3738.310072.8-0.5154375-1.1670631

64531003.152.1510010087.41.06087331-1.6006682

65100100100100100100282.563919382.0422319

665137.87.296.3930.710090-0.6061509-1.262203

672.313.617.311.25.626.917.7-3.09842031.16103527

69821004.135.246.1100900.74323502-1.6166055

7071.948.21.130.930.410046.5-0.7315845-0.9132592

7154.631.821.617.94541.190-0.6924507-0.3229265

7271.624.12.191.5932.710073.3-0.7512987-1.2134847

73100100100100100100903.152941971.14829359

7445.336.640.82815.665.653.5-1.07051840.46992615

755840.59.567.1221.910059.6-0.8716019-0.8000332

764.975.678.874.82.835.625.1-2.36453.26839967

7777.566.925.526.61009.952.20.274863930.4738433

782.495.635.235.91.2626.760.2-2.37268081.0314788

794.595.60.770.991.7112.726.8-3.30293440.58591128

8026.717.699.710015.95.6231.9-1.16312873.45768892

818.41.9917.220.632.3100100-1.2699173-0.7969928

8234.463.629.630.748.210061.7-0.1377268-0.1730437

8310045.914.713.656.759.31000.32708425-1.0242949

8454.521.214.59.1919.510071.4-1.0316007-0.7994097

8510010010010010010090.33.155792081.14396808

861001001001001001001003.247945611.00410999

8750.732.93.063.0629.7100100-0.6502958-1.5063163

8810010097.558.936.610084.81.903627170.63372198

891.737.10.20.7212.25.62100-2.5414438-0.4207296

90100100100100100311002.732842681.66300506

9114.58.831.051.189.89755.9-2.79778320.17412317

9210010010090.11005.6279.42.265971422.05112036

9310010017.817.614.31001000.82969862-1.3918476

9421.41001001001001001002.322029591.28748348

9577.655.44.583.5442.871.777.1-0.2792253-1.0456071

9666.559.610.17.16465.61000.16824998-1.1760092

975563.61.672.0950.510086.6-0.0676924-1.4675081

981001001001001001001003.247945611.00410999

991001003327.510010086.32.03094626-0.916687

10078.658.92.282.1147.123.382.1-0.5069768-0.7297348

1011001009.438.6859.41001001.29438947-1.7085396

10241.8100100100100551002.226406981.64365034

10348.459.34.314.1523100100-0.4034972-1.5216597

10427.664.21.391.5241100100-0.3883165-1.5649823

10640.41001.421.171001001001.01144629-1.7779813

1076.911.65.125.4616.75.6239.2-2.86459440.56704517

1087646.61.291.7837.410088.8-0.2008673-1.5264117

CORR1CORR2

DM0.44745202-0.1369412

DM Kit0.48719401-0.0949313

Vf-5min0.284741770.58898703

Vf-15min0.307006220.56843167

Tp-24h0.48388638-0.0437222

Ps kit0.27917705-0.35711

L minor0.27388343-0.415663

Sheet3


Correlaes de Pearson (p75No txico

10010074.776.310010026.39033.075 - 50Pouco txico

10.618.954.449.25.6212.830.46.31.050 - 25Txico

2431.91.741.0730.768.910061.32.225 - 2,5Muito txico

41.430.765.971.927.741.410029.815.0100No txico

10010074.776.310010026.39033.010 - 100Pouco txico

10.618.954.449.25.6212.830.46.31.01 - 10Txico

2431.91.741.0730.768.910061.32.275No txico75 - 50Pouco txico50 - 25Txico25 2,5Muito txico75Not ToxicBODBODBODBOD

75 - 50Sligtly ToxicCODCODCODCOD

50 - 25ToxicTSSTSSTSSTSS

25 - 2,5Very ToxicCyanideCyanideCyanideCyanide

ecoriver seminÁrio final

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