stereo d mo n e p qrgenesdev.cshlp.org/content/suppl/2009/04/16/23.8.939.dc1/... · 2009-04-16 ·...
TRANSCRIPT
A
B
C
D Stereo
F G H
I J
LK
E
M O
Q R
N
P
Reinders_ Supplementary Fig 1
0
5
10
15
epi 09 epi 07 epi 08 epi 42 epi 49 epi 53 epi 44 epi 12 epi 01 Col -0 epi 70
mg
FW /
plan
t
epi07
epi08
epi42
epi49 WT
epi70
epi09 ep
i53ep
i01ep
i44ep
i120
5
10
15Biomass
Reinders_ Supplementary Fig 2
0
20
40
60
80
100
9 m 61 50 16 45 36 42 7 18 C
Ger
min
atio
n ra
te (%
)
0
20
40
60
80
100
9 m 61 50 16 45 36 42 7 18 C
Ger
min
atio
n ra
te (%
)
0
20
40
60
80
100
9 m 61 50 16 45 36 42 7 18 C
Ger
min
atio
n ra
te (%
)
0
20
40
60
80
100
epi09 met epi16 epi61 epi50 epi45 Col
0
20
40
60
80
100
epi09 met epi16 epi61 epi50 epi45 Col
0
20
40
60
80
100
epi09 met epi16 epi61 epi50 epi45 Col
0
20
40
60
80
100
epi09 met epi16 epi61 epi50 epi45 Col
0 mM NaCl 150 mM NaCl
0
20
40
60
80
100
9 m 61 50 16 45 36 42 7 18 C
Ger
min
atio
n ra
te (%
)G
erm
inat
ion
(%)
20
40
60
80
100
0
Ger
min
atio
n (%
)
20
40
60
80
100
0
Ger
min
atio
n (%
)
20
40
60
80
100
0
Ger
min
atio
n (%
)
20
40
60
80
100
0
epi09
met1-3
epi61
epi50
epi16
epi45
epi36
epi42
epi07
epi18 WT
epi09
met1-3
epi61
epi50
epi16
epi45 WT
epi09
met1-3
epi61
epi50
epi16
epi45 WT
epi09
met1-3
epi61
epi50
epi16
epi45 WT
epi09
met1-3
epi61
epi50
epi16
epi45 WT
F8 epiRIL germination assays F9 epiRIL germination assays
2 days post sowing (dps)
4 dps
7 dps
9 dps
2 days post sowing (dps)
4 dps
7 dps
9 dps
A
B
C
D
Ger
min
atio
n (%
)G
erm
inat
ion
(%)
Ger
min
atio
n (%
)G
erm
inat
ion
(%)
80
E
F
G
H
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
80
100
0
20
40
60
100
0
80
epi09
met1-3
epi61
epi50
epi16
epi45
epi36
epi42
epi07
epi18 WT
epi09
met1-3
epi61
epi50
epi16
epi45
epi36
epi42
epi07
epi18 WT
Ger
min
atio
n (%
)
epi09
met1-3
epi61
epi50
epi16
epi45
epi36
epi42
epi07
epi18WT
Reinders_ Supplementary Fig 3
3
4
5
6
7
1 2 3 4 5 6 7 8 9 10 11cp
r5ep
i16
met1-3
epi44
epi60
epi26
NPRH WTep
i64 npr1
pad4
3.0
7.0
6.0
5.0
4.0
WTcp
r5ep
i16ep
i44ep
i60ep
i26NPRH
epi64
npr1
pad4
NP
RH
Wt-1
Pseudomonasresistance
Pseudomonasresistance
Pseudomonasresistance
bact
eria
l gro
wth
(Log
cfu
/ cm
2)
bact
eria
l gro
wth
(Log
cfu
/ cm
2)
bact
eria
l gro
wth
(Log
cfu
/ cm
2)
A
B
C
3.5
7.0
6.05.5
4.04.55.0
6.5
7.5
3.5
7.0
6.0
5.5
4.0
4.5
5.0
6.5
7.5
Sf-1
cpr5
Chi
-1
FI-1
Col
-0
Oy-
0
Ri-0
Cvi
-0
Go-
0
Blh-
1
npr1
Rsc
h-0
Nok
-1
pad4
Reinders_ Supplementary Fig 4
38 25
probe
RAP2.1 (AT1G46768)
1459
probe
1513
CMT3 (AT1G69770)
1781 79
9
4656
PHB (AT2G34710)
1862
probe
396
316
217
c5-a (AT5G42940)
411
889
probe
174
2420
c5-c (AT5G54440)
1184
3127
probe
probe
4215
3636
1140 150
160
1082
c5-b (chr5:20490000-20504000 bp)
1085
28
Reinders_ Supplementary Fig 5
chr1
chr2
chr3
chr4
chr5
chr1
chr2
chr3
chr4
chr5
chr1
chr2
chr3
chr4
chr5
Mbp
Mbp
Mbp
epi01
epi12
epi28
Reinders_ Supplementary Fig 6
Reinders_ Supplementary Fig 7
A
B
CA
B
trans
gres
sive
inte
rmed
iate
met
-like
Chr 2, met-like
Chr 3, WT-like Chr 5, WT-like
WT-
like
Chromosome-widetransgressive intermediate
Chr
omos
ome
1C
hrom
osom
e 2
Chr
omos
ome
3C
hrom
osom
e 4
Chr
omos
ome
5
trans
gres
sive
inte
rmed
iate
met
-like
WT-
like
Chr 4, met-like
Reinders_ Supplementary Fig 8
020406080
100
020406080
100
WTmetepi01
epi12
epi28
020406080
100
020406080
100
020406080
100
WT
epi01
epi12
epi28
met
5-m
ethy
lcyt
osin
e (%
)
CGCHGCHH
1 cytosines 51
chromosome 5
A
B bisulfite assay
100
0
100
0
100
0
100
0
100
0
19,984.8 19,985.0 19,985.2 19,985.4 19,985.6
AT5TE71885AT5G49260
(kb)
met-likeWT-like intermediate transgressive
Reinders_ Supplementary Fig 9
Reinders_ Supplementary Fig 10
MET CMT3 DRM2 DDM1 KYP IBM ROS1 PolIV 1a PolIV 1b PolIV 2a
MET
1
CMT3
DRM2
DDM1
KYP
IBM
1
ROS1
NRPD
1
NRPE
1
NRPD
2/NRPE
2
2.5
2.0
1.5
1.0
0.5
0
expr
essi
on le
vel r
elat
ive
to W
T
CACTA-silent epiRILs CACTA-active epiRILs
Reinders_ Supplementary Fig 11