crescimento epidermal
TRANSCRIPT
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Fator de Crescimento:
• São mediadores biológicos naturais que atuam sobre os processos de reparo e regeneração.
•Encontrados em vários tecidos em fase de cicatrização.
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Processo de Manufatura do Fator de Crescimento
Signal Sequence Growth Factor Gene
cDNA
① Gene Cloning
Vetor
E. coli
Vector
④ Secreted growth factors
③ Fermentation
② Transformation
⑤ Ultra Filtration
Column
⑥ Purification
Purified Growth factors
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A Caregen vem desenvolvendo dezenas de diferentes tipos de peptídeos, citoquinas, fator de crescimento, hormônios, neurotransmissores, imunomoduladores, como, EGF, IGF-1, bFGF, IL-10 e ainda fatores de crescimento semelhantes aos peptídeos humanos, como o Rejuline e CG-IDP2, todos com diversas atividades comparadas aos hormônios de crescimento intactos.
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A importância dos peptídedos e do fator decrescimento na regulação da expressão genética e do metabolismo celular é estabelecida na biologia da pele.Funções dos peptídeos e fatores de crescimento:
• Remove células epidermais danificadas;•Estimula a proliferação celular na pele e folículos capilares;•Repara e previne rugas além de minimizar as cicatrizes na pele;• Reduz e previne a celulite;• Previne a queda capilar;• Revitaliza e nutre as células epidermais e do couro cabeludo.
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A Caregen é líder mundial em cosmecêutica
A vantagem destes peptídeos como ingredientes cosméticos são:• Ação prolongada, devido a alta estabilidade, resultado da modificação da seqüência primária;• Penetração transdérmica por nano estrutura;• Alta pureza do produto.
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Fatores de crescimento:
Anti - Ageing Anti-RugasCG-EGF
(Epidermal Growth Factor)CG-EGF
(Epidermal Growth Factor)
CG-IGF-1(Insulin-like Growth Factor1)
CG-IGF-1(Insulin-like growth factor1)
CG-bFGF(basic Fibroblast G.F.)
CG-bFGF(basic Fibroblast G.F.)
CG-aFGF(acid Fibroblast G.F.)
CG-aFGF(acid Fibroblast G.F.)
CG-TRX(Thioredoxin)
CG-KGF(Keratinocyte G.F.)
CG-Somatotropin(Human Growth Factor)
CG-TGF-β3(Transforming G.F. β3)
CG-Thymosin β4Etc..
CG-Thymosin β4Etc..
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CG-EGFFator de Crescimento Epidermal
•INCI name: Human Oligopeptide-1
• Reduz e previne linhas e rugas pela ativação de novas células da pele;• Devolve a uniformidade no tom da pele, devolvendo vitalidade e energia;• Recupera a aparência jovial da pele;• Elimina cicatrizes e manchas da pele.
Conc. de uso: 1 – 5%
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CG-IGF 1Fator de Crescimento Isulin – Like - 1•INCI name: Human Oligopeptide-2
• Melhora a aparências de linhas e rugas de expressão;• Aumenta a produção de colágeno e elastina da pele e reduz manchas avermelhadas;• Possui um efeito redutor de gordura (drenagem) facial e corporal;• Estimula os folículos capilares a produzirem um cabelo mais denso e forte.
Conc. de uso: 1 – 5%
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PL ColⅠ FNColⅣ LN
ECM (Extracellular Matrix) Molecules
0
2
4
6
8
10
12 EGFIGF-1EGF+IGF-1
EGF and IGF-1 increase cell proliferationin to relation to ECM composition.
Efficacy Test Report Fo
ld In
crea
se In
[3H
] Thi
mid
ine
Inco
rpor
atio
n
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CG-bFGFFator de Crescimento Fibroblástico básico
•INCI name: Human Oligopeptide-3
• Reduz e previne linhas e rugas pela ativação de novas células da pele;;• Rejuvenecedor da pele, repara cicatriz e escoriaçãoes;• Melhora a elasticidade da pele;• Melhorara a circulação periférica, sendo indicado para desordens capilares.
Conc. de uso: 1 – 5%
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Rejuline
•INCI name: Acetyl Decapeptide-3
• Reduz e previne as linhas através da atividade das novas células formadas;
• Repara e melhora a cicatrização de ferimentos;
• Fortalece e modula a elasticidade da pele, por indução da síntese de colágeno e elastina;
• Regula a síntese de colágeno;
• Ação mais estável e prolongada que as proteínas nativas.
Conc. de uso: 1 – 5%
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The cell-cycle analysis and apoptotic retardation in human HacaT cell by peptides - Cell line : Human keratinocyte ( HacaT Cell) / Starvation : 24hrs / Peptide treatment : 72hrs - Control, IDP2 & Rejuline: 5 umole, Vitamin C: 5 umole- ProG1(M1) indicates the phase of apoptosis by FACS.
6. Apoptosis retardation by IDP2 & Rejuline
CG-IDP2
Control
Rejuline
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3.82.5
4.2 5.06.9 5.8
9.912.3
21.1
30.2
8.37.8
0
5
10
15
20
25
30
35
1 Day 2 Day 3 Day 4 Day 5 Day 6 Day
Control Rejuline (1 ug/ml)
Pro
cola
gen
(ng/
ml)
Rejuline age no incremento da síntese de pró - colágeno Haca T (Queratinócito humano)
HacaT Human Keratinocytes in 2% serum conditionProcollagen ELISA Kit analysis; Rejuline Peptide (1 ug/ml) treatment
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Áci
do H
ialu
rôni
co (n
g/m
l)
Aumento da síntese de ácido hialurônico CCD986SK(Fibroblasto Humano Cell Line)
Células fibroblasticas humanas em serum condition Hyaluronic Acid ELISA Kit analysis; 5umole of each peptides treatment for 72 hrs
117
159
75.7
176
50
90
130
170
210
Controle IDP-2 Rejuline Vit. C 50ppm
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Efficacy Test Report – Cell (NIH3T3) TestRejuline is modulates the skin elasticity by regulation ofcollagenase.
A. Control B. Rejuline Treated
Após 72hs de incubação com Rejuline 5ng/ml numa codição serum free.
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Antes Após 4 semanas
Efficacy Test Report - Tested by Ellead
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Antes Após 4 semanas
Efficacy Test Report - Tested by Ellead
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Mechanism of Growth Factors in Skin
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Epidermis TGF-α
Fibrin clot
Neutrophil Macrophage
PDGF BB
VEGF
FGF
PTGF-β1PDGF AB
Macrophage
Platelet plug
Blood vessel
Dermis
VEGF Neutrophil
FGF-2
Fat Fibroblast
IGF
PDGF
KGF
TGF-β1
FGF-2
Mecanismo do Fator de Crescimento na pele
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Manufacturing Process of Growth Factors
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Nano-Capsulation
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Topical Delivered System: Nano-capsulation
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IDP 2 have almost full activities compared to their intact growth factors.
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Efficacy Test Report -Mouse (C3H) Test
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Efficacy Test Report
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Ingredients Registered on CTFA-As of July 14, 2006
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EGF
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EGF ; Epidermal Growth Factor
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Efficacy Test Report
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Anti-hair loss complex solution have an additive effect on keratinocyte proliferation
III) In vitro test 3
A
B: 7 Days later
D: 7days laterC
IV) In vitro test 4
Mouse Skin Culture: A,B: Control
C,D: Complex treatment
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III. IGF-1 can induce Cell proliferation and Epidermis Thickness
cell proliferation (HacaT)
-20
0
20
40
60
80
100
control 100pg/m l 1ng/m l 10ng/m l 100ng/m l 1ug/m l
IGF-1
%
IGF-1 induced cell proliferation in Human Keratinocyte cells(Hacat) as a dose dependant manner which
was observed with SRB Assay.
1) Cell growth assay (SRB)
Outer layer
Epidermis
dermis
2) IGF-1 induces Epidermal Thickness
a) Control b) IGF-1 Treated(1ug/ml)
Nano-capsulayted IGF-1 induced epidermis thickness after 1 week treatment in mouse skin.
Outer layer
Epidermis
dermis
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Control
Control (HA only)
Copper Peptide
Hair Complex
V) In vitro test 5: Mouse Wound Healing 4 days later
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IGF-1 ; Insulin-like Growth Factor-1
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IGF -1
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Efficacy Test Report
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bFGF ; basic Fibroblast Growth Factor
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bFGF
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bFGF modulates theskin elasticity by regulation of collagenase.
(ng/ml)
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Rejuline
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Rejuline
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Rejuline is modulates the skin elasticityby regulation of
collagenase
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VI) In vitro test 6: Mouse Hair Growth test
Hair growth stimulation (Mouse C3H / HeN, 4month Old)
Control
After 1day After 14days After 25days
Anti-hair losscomplex
After 1day After 14days After 25days
Black
Skin
Red
Skin
Red
Skin
Black
Skin
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- Apr. 18. 06 -
>>>
# 1. Before After (1weeks)
- Apr. 25. 06 - - May. 02. 06 -
>>>
After (2weeks)
Clinical test for Healthier hair and scalp_ # 1
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