formato per il curriculum vitae · e-mail [email protected] nazionalità italiana data di...

FORMATO

EUROPEO

PER IL

CURRICULUM

VITAE

INFORMAZIONIPERSONALI

Nome

IANNELLO DANIELA

Telefono

+39 (090) 221 3303

Fax

3302

Curriculum Vitae - IANNELLO Daniela https://www.polime.it/curriculum/view_cv.php?id_curriculum=116

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E-mail

[email protected]

Nazionalità

ITALIANA

Data di Nascita

14/11/1953

ESPERIENZALAVORATIVA

Date (da - a)

01/04/1978 -

Nome e indirizzo deldatore di lavoro

UNIVERSITÀ DI MESSINA, FACOLTÀ DI MEDICINA ECHIRURGIA

Tipo di azienda o settore

ISTRUZIONE

Tipo di impiego

PROF. ASSOCIATO

Principali mansioni e


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responsabilità

DALL'ANNO ACCADEMICO 1992-1993 È PROFESSOREASSOCIATO DI MICROBIOLOGIA PRESSO LA FACOLTÀDI MEDICINA E CHIRURGIA DELL’UNIVERSITÀ DEGLISTUDI DI MESSINA DAL 1987 AL 1992 PROFESSOREASSOCIATO DI MICOLOGIA PRESSO LA FACOLTÀ DIMEDICINA E CHIRURGIA DELL’UNIVERSITÀ DEGLISTUDI DI MESSINA RICERCATRICE CONFERMATADALL' 1/8/1980 PRESSO LA FACOLTÀ DI MEDICINA ECHIRURGIA DELL’UNIVERSITÀ DEGLI STUDI DIMESSINA HA PRESO SERVIZIO DALL’1 APRILE 1978COME ASSISTENTE INCARICATO SUPPLENTE PRESSOL’ISTITUTO DI MICROBIOLOGIA DELLA FACOLTÀ DIMEDICINA E CHIRURGIA DELL’UNIVERSITÀ DEGLISTUDI DI MESSINA. DALL'A.A.2006 DOCENTE NELDOTTORE DI RICERCA IN "IGIENE APPLICATA ALLAVALUTAZIONE E GESTIONE DEL RISCHIOMICROBIOLOGICO ALIMENTARE ED AMBIENTALE.NEGLI A. A. 80/81 E 81/82 DOCENTE DI TECNICHEIMMUNOLOGICHE IN VIROLOGIA NELLA SCUOLA DISPECIALIZZAZIONE IN "VIROLOGIA" DALL'A.A. 87/88DOCENTE DI PROTOZOOLOGIA E MICOLOGIA E ,SUCCESSIVAMENTE, DI SIEROLOGIA NELLA SCUOLADI SPECIALIZZAZIONE IN MICROBIOLOGIA APPLICATADELLA FACOLTÀ DI SCIENZE DELLA UNIVERSITÀ DIMESSINA DALL'A.A. 2005/2005 DOCENTE DIMICROBIOLOGIA E MICROBIOLOGIA CLINICA NELLASCUOLA DI SPECIALIZZAZIONE DI "MALATTIEDELL'APPARATO RESPIRATORIO"

Date (da - a)

01/04/1978 -

Nome e indirizzo deldatore di lavoro

POLICLINICO UNIVERSITARIO, FACOLTÀ DI MEDICINAE CHIRURGIA

Tipo di azienda o settore

SANITÀ


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Tipo di impiego

RESPONSABILE U.O.C. DI MICROBIOLOGIA CLINICA

Principali mansioni eresponsabilità

DAL 1 MAGGIO 2004 È RESPONSABILE DELLA U.O.C.DIMICROBIOLOGIA CLINICA DELLA A.O.U. G. MARTINONELL’AMBITO DELLA QUALE SVOLGE LA SUA ATTIVITÀDIAGNOSTICA E DIDATTICA, NONCHÉ LE FUNZIONI DICOORDINAMENTO E GESTIONE RICHIESTE DALL’INCARICO CHE LE È STATO AFFIDATO. COMPONENTEDEL COMITATO INFEZIONI OSPEDALIERE DALLA DATADI ASSUNZIONE HA SVOLTO MANSIONI DI AIUTO

ISTRUZIONE EFORMAZIONE

Date (da - a)

- 18/11/1981

Nome e tipo di istituto diistruzione o formazione

UNIVERSITÀ DEGLI STUDI DI FIRENZE, FIRENZE -ITALIA

Titolo di Studio

SPEC.NE IN ALLERGOLOGIA E IMMUNOLOGIA CLINICA

Qualifica conseguita

SPECIALISTA IN ALLERGOLOGIA E IMMUNOLOGIA


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CLINICA

Livello nellaclassificazione nazionale

70/70 CON LODE

Date (da - a)

- 29/07/1977


UNIVERSITÀ DI MESSINA , MESSINA - ITALIA

Titolo di Studio

LAUREA IN MEDICINA E CHIRURGIA


DOTT. IN MEDICINA E CHIRURGIA


110/110 CON LODE

Date (da - a)

- 23/07/1971


ISTITUTO PARIFICATO S. GIUSEPPE, CATANIA - ITALIA


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Titolo di Studio

MATURITA' CLASSICA


MATURITÀ CLASSICA


60/60

PUBBLICAZIONI

Titolo

AN EXOPOLYSACCHARIDE PRODUCED BYGEOBACILLUS THERMODENITRIFICANS STRAIN B3-72:ANTIVIRAL ACTIVITY ON IMMUNOCOMPETENT CELLS.

Autori

ARENA ADRIANA, CONCETTA GUGLIANDOLOB,GIOVANNA STASSI, BERNADETTE PAVONE, DANIELAIANNELLO, GIUSEPPE BISIGNANOC, TERESA LUCIANAMAUGERI

Abstract

THE IMMUNOMODULATORY AND ANTIVIRAL EFFECTSOF AN EXTRACELLULAR POLYSACCHARIDE (EPS-2),PRODUCED BY A STRAIN OF GEOBACILLUSTHERMODENITRIFICANS ISOLATED FROMASHALLOWMARINE VENT OF VULCANO ISLAND(ITALY),WERE EVALUATED. IN THE PRESENT STUDY,


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WE SHOW FOR THE FIRST TIME THAT EPS-2TREATMENT HINDER HSV-2 REPLICATION IN HUMANPERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC)BUT NOT IN WISH CELLS. IN FACT, HIGH LEVELS OFIFN-, IL-12, IFN-, TNF-, IL-18 WERE DETECTED INSUPERNATANTS OF EPS-2 TREATED PBMC.MOREOVER, THIS EFFECT WAS DOSE-DEPENDENT.TAKEN TOGETHER, OUR RESULTS CONFIRM THAT THEIMMUNOLOGICAL DISORDERS DETERMINED BY HSV-2COULD BE PARTIALLY RESTORED BY TREATMENTWITH EPS-2.

Anno pubblicazione eriferimenti

IMMUNOLOGY LETTERS, VOL. 123; P. 132-137, ISSN:0165-2478, DOI: 10.1016/J.IMLET.2009.03.001ANNO: 2009 - ISBN:

Titolo

FACILE BIOCATALYTIC ACCESS TO9-FLUORENYLMETHYL POLYGLYCOSIDES:EVALUATION OF ANTIVIRAL ACTIVITY ONIMMUNOCOMPETENT CELLS.

Autori

A. TRAMICE, ARENA ADRIANA, A. DE GREGORIO, R.OTTANÀ, R.MACCARI, B.PAVONE, N.ARENA,D.IANNELLO, M.G.VIGORITA, A.TRINCONE

Abstract

THE BIOLOGICAL ACTIVITIES OF A SERIES OF MONO-AND OLIGOSACCHARIDES (B-XYLOSIDES ANDA-GLUCOSIDES) OF 9-FLUORENYLMETHANOL WEREINVESTIGATED TOGETHER WITH MONO-B-GALACTOSIDE AND B-GLUCOSIDE OF THISAGLYCONE, PRODUCED BY BIOCATALYTIC ROUTES.BY USING MARINE GLYCOSIDE HYDROLASES ANDINEXPENSIVE DONORS SUCH AS MALTOSE OR XYLAN,ACCESS TO MONO-A-GLUCOSIDE OR MONO-B-XYLOSIDE OF 9-FLUORENYLMETHANOL WAS


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OBTAINED. ADDITIONALLY, INTERESTINGPOLYGLYCOSIDE DERIVATIVES WERE ISOLATED.BIOLOGICAL TESTING INDICATED THAT IN VITROTREATMENT WITH THESE CARBOHYDRATEDERIVATIVES MAY INFLUENCE THE BALANCE OFCYTOKINES IN THE ENVIRONMENT OF HUMANPERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC),RESTRICTING THE HARMFUL EFFECT OF HERPESSIMPLEX TYPE 2 REPLICATION. IN FACT, THESECARBOHYDRATE DERIVATIVES TESTED IN WISHCELLS DID NOT SHOW ANY SIGNIFICANT ANTIVIRALACTIVITY.


CHEMMEDCHEM, ISSN: 1860-7179, DOI:10.1002/CMDC.200800086ANNO: 2008 - ISBN:

Titolo

IN VITRO TREATMENT WITH KILLED HELICOBACTERPYLORI DOWNREGULATES THE PRODUCTION OFRANTES BY PBMC.

Autori

G.STASSI, B. PAVONE, A. SPERANZA, D. GAZZARA, G.B.COSTA, D.IANNELLO, ARENA ADRIANA

Abstract

THE MECHANISMS BY WHICH H. PYLORI COLONIZESAND PERSISTS WITHIN THE GASTRIC MUCOSA AREPOORLY UNDERSTOOD. THE GASTRIC IMMUNERESPONSE OBSERVED “IN VIVO”, DURING H. PYLORIINFECTION, IS CHARACTERIZED BY A POLARIZATIONOF TH1 CELL TYPE THAT SEEMS TO BE RESPONSIBLEOF GASTRIC PATHOLOGY. THE PURPOSE OF THISSTUDY WAS TO TEST THE DIRECT EFFECT OF H.PYLORI CAGA+/VACA+ (LIVE AND/OR GENTAMICIN-KILLED) ON HUMAN PBMC IN ORDER TO EVALUATETHE PRODUCTION OF RANTES “IN VITRO”.


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FURTHERMORE, WE EVALUATED THE POSSIBLERELATIONSHIP BETWEEN RANTES RELEASE AND THEPRESENCE OF IL-12 AND IFN- IN SUPERNATANTS OFTHE SAME CELLS. IN THE PRESENT STUDY, WESHOWED FOR THE FIRST TIME THAT THE LOWAMOUNT OF RANTES IN SUPERNATANTS OF PBMCINCUBATED WITH KILLED H. PYLORI IS LINKED, ATLEAST IN PART, TO THE INHIBITION OF IL-12 ANDIFN- RELEASE.


JOURNAL OF CHEMOTHERAPY, VOL. 20; P. 48-52, ISSN:1120-009XANNO: 2008 - ISBN:

Titolo

IMPAIRED ANTIVIRAL ACTIVITY OF MONOCYTES FROMPATIENTS ON HEMODIAFILTRATION.

Autori

ARENA ADRIANA, COPPOLINO G, NOSTRO L, PAVONEB, BONVISSUTO G, CAMPO S, IANNELLO D, BONINA L,BUEMI M

Abstract

BACKGROUND: THE AIM OF OUR STUDY WAS TODETERMINE WHETHER INTERMITTENTHEMODIAFILTRATION (HDF) LEADS TO ANALTERATION IN MONOCYTE ANTIVIRAL ACTIVITY ASWELL AS IN THE IN VITRO RELEASE OF CYTOKINESSUCH AS INTERLEUKIN-12 (IL- 12), TUMOR NECROSISFACTOR-Α (TNF-Α) AND INTERFERON-Α (IFN-Α) BY THESAME CELLS. METHODS: WE ENROLLED 25 PATIENTSUNDERGOING HDF FOR 3.5-4 HOURS 3 TIMES A WEEK(12 MEN, 13 WOMEN; MEAN AGE 58 ± 6.7 YEARS) AND25 HEALTHY DONORS (ND) (12 MEN, 13 WOMEN; MEANAGE 57 ± 8 YEARS). MONOCYTES FROM PERIPHERALBLOOD MONONUCLEAR CELLS WERE ISOLATED WITHA MONOCYTE ISOLATION KIT II. MONOCYTIC CELLS


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WERE INFECTED WITH HERPES SIMPLEX VIRUS TYPE2 (HSV-2). CYTOKINES WERE ASSAYED INSUPERNATANTS. RESULTS: THE IN VITRO ANTIVIRALACTIVITY OF MONOCYTES FROM HDF PATIENTS WASSIGNIFICANTLY IMPAIRED WITH RESPECT TO ND.FURTHERMORE, MONOCYTES FROM POST-HDFPATIENTS WERE MORE PRONE TO VIRAL INFECTION.LIPOPOLYSACCHARIDE (LPS) STIMULATION INDUCEDSIGNIFICANT VIRAL INHIBITION ONLY IN MONOCYTESFROM NDS (P<0.05). THE CYTOKINE PATTERN (TNF-Α,IFN-Α AND IL-12) IN MONOCYTES STIMULATED WITHLPS WAS MARKEDLY INHIBITED IN HDF PATIENTSCOMPARED WITH ND (P<0.05). A BASAL PRODUCTIONOF TNF-Α WAS FOUND IN MONOCYTES FROM PRE-HDFAND POST-HDF PATIENTS. NO IFN-Α PRODUCTIONWAS FOUND IN LPS-STIMULATED AND HSV-2-INFECTED MONOCYTES FROM PRE-HDF AND POST-HDF PATIENTS. IL-12 PRODUCTION APPEAREDSIGNIFICANTLY DECREASED AFTER HDF IN ALLEXPERIMENTAL CONDITIONS (P<0.05). CONCLUSIONS:THE SIGNIFICANT INCREASE OF VIRAL REPLICATIONIN MONOCYTES FROM HDF PATIENTS COMPAREDWITH HEALTHY DONORS COULD BE RELATED TO ASIGNIFICANT REDUCTION OF CYTOKINEPRODUCTION. MOREOVER, THE DIALYTIC SESSIONINFLUENCED THE INTRINSIC ANTIVIRAL ACTIVITY OFMONOCYTES, FAVORING VIRAL REPLICATION.


JOURNAL OF NEPHROLOGY, VOL. 20; P. 560-567, ISSN:1121-8428ANNO: 2007 - ISBN:

Titolo

MODULATION OF GRO-ALPHA AND TNF-ALPHAPRODUCTION BY PERIPHERAL BLOODMONONUCLEAR CELLS TREATED WITH KILLEDHELICOBACTER PYLORI

Autori

STASSI G, CASCIO A, IARIA C, GAZZARA D, COSTA GB,


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IANNELLO D, ARENA ADRIANA

Abstract

GRO- ALPHA SEEMS TO PLAY AN IMPORTANT ROLE INRECRUITING AND ACTIVATING NEUTROPHILS DURINGHELICOBACTER PYLORI INFECTION. IN THE PRESENTSTUDY, WE EXAMINED HOW TREATMENT WITH KILLEDH. PYLORI OR/AND LIVE H. PYLORI MAYDIFFERENTIALLY INFLUENCE THE “IN VITRO” GRO-ALPHA AND TNF-ALPHA RELEASE BY PERIPHERALBLOOD MONONUCLEAR CELLS (PBMC). THE AMOUNTSOF TNF-ALPHA AND GRO-ALPHA PRODUCED BY PBMCAFTER STIMULATION WITH LIVE H. PYLORI WEREHIGHER THAN THOSE PRODUCED AFTERSTIMULATION WITH A COMBINATION OF KILLED ANDLIVE H. PYLORI AND THE LATTER WERE HIGHER THANTHOSE PRODUCED AFTER STIMULATION WITH KILLEDH. PYLORI. IN CONCLUSION, THE TREATMENT OFPERIPHERAL BLOOD MONONUCLEAR CELLS WITHKILLED H. PYLORI DOWN-REGULATES THEPRODUCTION OF GRO-ALPHA. TAKEN TOGETHER,OUR DATA DEMONSTRATE THAT TREATMENT WITHKILLED H. PYLORI COULD REPRESENT ANINNOVATIVE APPROACH DURING GASTRIC INFECTIONSUPPORTED BY H. PYLORI.


EUROPEAN JOURNAL OF INFLAMMATION, VOL. 5; P.83-88, ISSN: 1721-727XANNO: 2007 - ISBN:

Titolo

ANTIVIRAL AND IMMUNOREGULATORY EFFECT OF ANOVEL EXOPOLYSACCHARIDE FROM A MARINETHERMOTOLERANT BACILLUS LICHENIFORMIS.

Autori

ARENA A, MAUGERI TL, PAVONE B, IANNELLO D.,GUGLIANDOLO C, BISIGNANO G


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Abstract

EPS-1 IS A NOVEL EXTRACELLULARPOLYSACCHARIDE PRODUCED BY A STRAIN OFTHERMOTOLERANT BACILLUS LICHENIFORMIS,ISOLATED FROM A SHALLOW MARINE HOT SPRING OFVULCANO ISLAND (ITALY). IN THIS PAPER, ANTIVIRALAND IMMUNOMODULATORY EFFECTS OF EPS-1 WEREEVALUATED. IT WAS FOUND THAT EPS-1 TREATMENTIMPAIRED HSV-2 REPLICATION IN HUMAN PERIPHERALBLOOD MONONUCLEAR CELLS (PBMC) BUT NOT INWISH CELLS. SINCE SEVERAL CYTOKINES MODULATETHE IMMUNE RESPONSE TO VIRUSES, TH1- AND TH2-TYPE CYTOKINES WERE ASSAYED IN SUPERNATANTSOF PBMC IN DIFFERENT EXPERIMENTAL CONDITIONS.EPS-1 INDUCED IL-12, IFN-G, IFN-A, TNF-A AND IL-18,BUT NOT IL-4. THUS, THE ANTIVIRAL EFFECT OF EPS-1ON PBMC SEEMS TO BE RELATED TO THE PATTERNOF CYTOKINES INDUCED.


INTERNATIONAL IMMUNOPHARMACOLOGY, VOL. 6; P.8-13, ISSN: 1567-5769ANNO: 2006 - ISBN:

Titolo

DIFFERENTIAL INDUCTION OF TNF ALPHA AND IL-18 INHUMAN PERIPHERAL BLOOD MONONUCLEAR CELLSINFECTED WITH LEISHMANIA MAJOR OR LEISHMANIADONOVANI.

Autori

IANNELLO D., ARENA A, BUEMI C, CALAPAI M, STASSIG, GAZZARA D, MASTROENI P

Abstract

SEVERAL CYTOKINES ARE INVOLVED IN THE HOST


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RESPONSE TO LEISHMANIA . HOWEVER,THE ROLEPLAYED BY CYTOKINES DURING INFECTION WITHDIFFERENT SPECIES OF LEISHMANIA IS NOTUNIVOCAL.IN THIS WORK, THE PRODUCTION OFTUMOR NECROSIS FACTOR ALPHA (TNF ) ANDINTERLEUKIN 18 (IL-18) DURING INTERACTION OFHUMAN PHAGOCYTES WITH LEISHMANIA MAJOR OR L.DONOVANI WAS COMPARATIVELY INVESTIGATED.PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC)AND MONOCYTES FROM HEALTHY DONORS WEREUSED. THE RELEASE OF TNF AND IL-18 DURINGINFECTION OF CELLS WITH DIFFERENT SPECIES OFLEISHMANIA "IN VITRO" WAS EVALUATED. L.DONOVANI INDUCED IN BOTH PBMC AND MONOCYTESSIGNIFICANTLY MORE TNF AND IL-18 WITHRESPECT TO L. MAJOR. THE AMOUNTS OF TNFRELEASED BY PBMC WERE ALWAYS SIGNIFICANTLYHIGHER THAN THOSE RELEASED BY MONOCYTES OFTHE SAME DONORS.


NEW MICROBIOLOGICA, VOL. 26; P. 399 - 404, ISSN:1121-7138ANNO: 2003 - ISBN:

Titolo

TREATMENT OF PBMC WITH KILLED HELICOBACTERPYLORI SUBVERTS THE ENVIRONMENT OFINFLAMMATORY CYTOKINES.

Autori

STASSI G, ARENA A, SPERANZA A, IANNELLO D.,MICELI M, MASTROENI P

Abstract

IT IS WELL KNOWN THAT INFLAMMATION INDUCED BYHELICOBACTER PYLORI IS CHARACTERIZED BY THELOCAL PRODUCTION OF CYTOKINES ANDCHEMOKINES. IN THE PRESENT STUDY, WE ANALYSE


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THE KINETICS OF MCP-1, IL-12 AND IL-4 INDUCTIONDURING THE INTERACTION OF PERIPHERAL BLOODMONONUCLEAR CELLS WITH KILLED AND/OR LIVE H.PYLORI. OUR RESULTS DEMONSTRATE THAT LIVE H.PYLORI DOES NOT INDUCE IL-4 RELEASE WHEREASIT STIMULATES MCP-1 AND IL-12 PRODUCTION. INADDITION, THE NEUTRALIZATION OF IL-12 WITHMONOCLONAL ANTIBODIES DETERMINES A LOWERMCP-1 RELEASE. THESE DATA DEMONSTRATE THATMCP-1 PRODUCTION IS IN PART SUPPORTED BY IL-12INDUCED BY LIVE H. PYLORI. ON THE CONTRARY,KILLED H. PYLORI STIMULATES THE IL-4 BUT NOTMCP-1 AND IL-12 PRODUCTION. THE COMBINEDTREATMENT WITH KILLED AND LIVE H. PYLORIUPREGULATES THE IL-4 PRODUCTION AND AT THESAME TIME DOWNREGULATES IL-12 AND MCP-1PRODUCTION.



Titolo

MODULATORY EFFECT OF HHV-6 ON MCP-1PRODUCTION BY HUMAN MONOCYTES.

Autori

ARENA A, STASSI G, SPERANZA A, IANNELLO D.,MASTROENI P

Abstract

CHEMOKINES REPRESENT A LARGE FAMILY OFPROINFLAMMATORY PROTEINS THAT ORCHESTRATELEUKOCYTE TRAFFICKING TO SITES OF VIRALINFECTION. HUMAN HERPES VIRUS TYPE 6 (HHV-6) ISA TYPICAL IMMUNOSUPPRESSIVE AGENT, ASSUGGESTED BY ITS TROPISM. IN THIS STUDY THEPRODUCTION OF MONOCYTE CHEMOATTRACTANT


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PROTEIN –1 (MCP-1) AND INTERLEUKIN-10 (IL-10) BYHUMAN PERIPHERAL BLOOD MONOCYTES WASEVALUATED DURING HHV-6 INFECTION. OUR RESULTSDEMONSTRATE THAT HHV-6 INFECTION TRIGGERSMONOCYTES TO RELEASE MCP-1 AND IL-10. THEADDITION OF EXOGENOUS RECOMBINANT MCP-1UPREGULATES THE RELEASE OF EXTRACELLULARVIRUS, WHEREAS DOES NOT INFLUENCE THEPERCENTAGE OF VIRAL-ANTIGEN POSITIVE CELLS.FURTHERMORE, THE ADDITION OF MONOCLONALANTIBODIES ANTI-IL-10 DOWN-REGULATES MCP-1RELEASE INDUCED BY HHV-6.THESE FINDINGSINDICATE THAT IL-10 AND MCP-1 PRODUCTION WASCLOSELY RELATED AND THAT THE MARKED AMOUNTSOF MCP-1 WERE SUPPORTED NOT ONLY BY VIRUSBUT ALSO BY VIRUS-INDUCED IL-10.



Titolo

DIFFERENT MODULATION BY LIVE OR KILLEDHELICOBACTER PYLORI ON CYTOKINE PRODUCTIONFROM PERIPHERAL BLOOD MONONUCLEAR CELLS.

Autori

STASSI G, ARENA A, SPERANZA A, IANNELLO D.,MASTROENI P

Abstract

THE MECHANISMS BY WHICH H. PYLORI COLONIZESAND PERSISTS WITHIN THE GASTRIC MUCOSA AREPOORLY UNDERSTOOD. THE INDUCTION ANDMAINTENANCE OF GASTRIC INFLAMMATION APPEARTO DEPEND ON THE COMPLEX INTERACTIONBETWEEN A NUMBER OF CYTOKINES ANDCHEMOKINES. THE GASTRIC IMMUNE RESPONSE


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OBSERVED "IN VIVO", DURING H. PYLORI INFECTION,IS CHARACTERIZED BY A POLARIZATION OF TH1 CELLTYPE THAT SEEMS TO BE RESPONSIBLE FORGASTRIC PATHOLOGY. THE PURPOSE OF THIS STUDYWAS TO TEST THE DIRECT EFFECT OF H. PYLORI(LIVE OR GENTAMICIN-KILLED) ON HUMAN PBMC INORDER TO EVALUATE THE "IN VITRO" TH1-TH2BALANCE BY MONITORING IL-18, IFNGAMMA AND IL-10PRODUCTION. THIS STUDY DEMONSTRATES FOR THEFIRST TIME THAT "IN VITRO" PRETREATMENT WITHGENTAMICIN-KILLED H. PYLORI OF PBMC, FOLLOWEDBY INFECTION WITH LIVE BACTERIA,DOWNREGULATES THE PRODUCTION OFINFLAMMATORY CYTOKINES SUCH AS IL-18 ANDIFNGAMMA OUR RESULTS PROVIDE A POSSIBLESTRATEGY TO RESTORE THE IMMUNOLOGICALDISORDERS DETERMINED BY H. PYLORI INFECTION.



Titolo

ROLE OF IL-18 IN PBMC INFECTED WITH HUMANHERPES VIRUS TYPE 6.

Autori

ARENA A., IANNELLO D., GAZZARA D., SPERANZAA.,BONINA L., MASTROENI P.

Abstract

OBJECTIVE. IL-18 PRODUCTION REPRESENTS ACRITICAL STEP IN THE POLARIZATION OF THE TH1IMMUNE RESPONSE. HHV-6 POSSESSES A PECULIARTROPISM FOR IMMUNOCOMPETENT CELLS. TOUNDERSTAND THE RELATIONSHIPS AMONGIMMUNOCOMPETENT CELLS, HHV-6 AND CYTOKINES,THE ROLE OF IL-18 DURING INFECTION OF PBMC


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WITH HHV-6 WAS EVALUATED. METHODS. PBMC WEREOBTAINED FROM HEALTHY DONORS, AFTERCENTRIFUGATION OF HEPARINIZED VENOUS BLOODOVER FICOLL-HYPAQUE GRADIENT.SUPERNATANTSFROM PBMC, WERE ANALYSED FOR THE PRESENCEOF CYTOKINES BY IMMUNOENZIMATIC METHOD.INORDER TO STUDY THE EFFECTS OF EXOGENOUSRHIL-18 ON HHV-6 REPLICATION WERE ANALYSED THENUMBER OF CELLS EXPRESSING VIRAL ANTIGENS BYIFA AND THE AMOUNT OF EXTRACELLULAR VIRUS BYF.F.U./ML. RESULTS.NO BASAL PRODUCTION OF IL-18WAS FOUND IN SUPERNATANTS OF UNSTIMULATEDPBMC. APPRECIABLE AMOUNTS OF THE CYTOKINESWERE PRODUCED BY LPS-STIMULATED PBMC. HHV-6INFECTION OF LPS-TREATED PBMCDOWNREGULATED IL-18 PRODUCTION. IT WAS FOUNDTHAT THE ADDITION OF RHIL-18 TO HHV-6 INFECTEDPBMC DOWNREGULATED THE PERCENTAGE OF HHV-6ANTIGEN POSITIVE CELLS AND THE RELEASE OFEXTRACELLULAR VIRUS. CONCLUSION. IMPAIRMENTOF IL-18 RELEASE, WHICH IS INVOLVED IN THEINDUCTION OF ANTIVIRAL CYTOKINES SUCH AS IFN ,COULD REPRESENT A STRATEGY OF THE VIRUS TOEVADE THE IMMUNE RESPONSE OF THE HOST, THUSESTABLISHING ITS OWN PERSISTENT INFECTION.


INTERVIROLOGY 44:250-254ANNO: 2001 - ISBN:

Titolo

ROLE OF IL-15ON MONOCYTIC RESISTANCE TOHUMAN HERPESVIRUS 6 INFECTION.

Autori

ARENA A., MERENDINO R.A., BONINA L., IANNELLO D.,STASSI G., MASTROENI P.

Abstract


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INTERLEUKIN-15 (IL-15) IS A CYTOKINE THATPOSSESSES A VARIETY OF BIOLOGICAL FUNCTIONS,INCLUDING STIMULATION AND MAINTENANCE OFCELLULAR IMMUNE RESPONSES. RECENTLY, IT HASBEEN DEMONSTRATED THAT HUMAN HERPES VIRUSTYPE 6 (HHV-6) ENHANCES NK ACTIVITY OF HUMANPBMC BY INDUCING IL-15. HHV-6 IS A TYPICALIMMUNOSUPPRESSIVE AGENT, AS SUGGESTED BY ITSTROPISM FOR BOTH CD4+ AND CD8+ T CELLS, BCELLS, MONOCYTES/MACROPHAGES,MEGAKARYOCYTES AND NK CELLS. MOREOVER,SEVERAL STUDIES HAVE INDICATED THATMONONUCLEAR PHAGOCYTE RESISTANCE TO VIRUSINFECTION IS INFLUENCED BY THE CELLULARDIFFERENTIATION STATE. THIS PAPER DESCRIBESTHE EFFECT OF PRETREATMENT "IN VITRO" WITHIL-15 ON THE RESISTANCE OF HUMAN MONOCYTES(HM) TO HHV-6 INFECTION. OUR RESULTSDEMONSTRATE THAT UNDIFFERENTIATED HM WEREHIGHLY RESISTANT TO HHV-6 INFECTION, WHEREASHM PRETREATED WITH HUMAN RECOMBINANT IL-15SHOWED AN INCREASED PERMISSIVENESS FORHHV-6 INFECTION. THIS PERMISSIVENESS WASCHARACTERISED BY HIGHER RELEASE OFEXTRACELLULAR VIRUS AS WELL AS AN INCREASEDPERCENTAGE OF ANTIGEN POSITIVE CELLS.MOREOVER, WE EVALUATED IL-15 PRODUCTIONAFTER THE ADDITION OF HHV-6 TO MONOCYTESPRECULTURED IN DIFFERENT EXPERIMENTALCONDITIONS. OUR DATA INDICATE THAT HHV-6-INDUCED IL-15 PRODUCTION BY HUMAN MONOCYTESIS NOT AFFECTED BY THE CONDITION OF "IN VITRO"PRECULTIVATION/DIFFERENTIATION. FURTHERMORE,THE NEUTRALIZATION OF IL-15 INDUCED BY HHV-6 INDIFFERENTIATED MONOCYTES DID NOT AFFECTVIRAL REPLICATION. THESE FINDINGS SUGGESTTHAT IL-15 ACTS ONLY ON THE MECHANISMS OFCELLULAR DIFFERENTIATION, RENDERING HM MORESUSCEPTIBLE TO HHV-6 INFECTION, WITHOUTINTERFERING WITH VIRUS REPLICATION.


NEW MICROBIOLOGICA, VOL. 23; P. 105-112, ISSN:1121-7138ANNO: 2000 - ISBN:


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Titolo

GRANULOCYTE-MACROPHAGE COLONY STIMULATINGFACTOR MODULATES THE PRODUCTION OF TNFA BYDIFFERENTIATED U937 CELLS INFECTED WITHLEISHMANIA MAJOR.

Autori

ARENA A., CAPOZZA A.B., MARINO F., ZUMMO S.,IANNELLO D., MASTROENI P.

Abstract

IN THIS WORK, THE PRODUCTION OF TUMORNECROSIS FACTOR ALPHA (TNF ALPHA) DURINGINTERACTION OF HUMAN PHAGOCYTES WITH THEINTRACELLULAR PARASITE LEISHMANIA MAJOR WASFURTHER INVESTIGATED. THE HUMAN MONOCYTICCELL LINE U937, DIFFERENTIATED WITH ACOMBINATION OF 1 ALPHA, 25 DIHYDROXYVITAMIN D3(VD) AND RETINOIC ACID (RA), OR WITHGRANULOCYTE MACROPHAGE COLONY STIMULATINGFACTOR (GM-CSF) WAS USED. DIFFERENTIATED U937CELLS WERE INFECTED WITH LEISHMANIA MAJORPROMASTIGOTES, AND TNF ALPHA WAS ASSAYED INCELL CULTURE SUPERNATANTS. IT WAS FOUND THATTHE CYTOKINE WAS PRODUCED ONLY BY U937 CELLSDIFFERENTIATED WITH VD/RA AND FURTHERINCUBATED WITH GM-CSF AND LPS OR INTERFERONGAMMA (IFN GAMMA). L. MAJOR INDUCED TNF ALPHAPRODUCTION ONLY IN THE PRESENCE OF GM-CSF. NODIRECT RELATIONSHIP WAS FOUND, HOWEVER,BETWEEN PRODUCTION OF TNF ALPHA ANDRESISTANCE OF DIFFERENTIATED U937 CELLS TOINFECTION WITH L. MAJOR.


NEW MICROBIOLOGICA, VOL. 22; P. 31-39, ISSN:1121-7138ANNO: 1999 - ISBN:


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Titolo

ALTERED CYTOKINE PRODUCTION AFTER HUMANHERPES VIRUS TYPE 6 INFECTION.

Autori

ARENA A, LIBERTO MC, IANNELLO D., CAPOZZA AB

Abstract

SEVERAL STRATEGIES ALLOW VIRUSES TO ELUDETHE SURVEILLANCE OF THE IMMUNE SYSTEM AND TOESTABLISH PERSISTENT INFECTION IN THE HOST.ONE OF SUCH MECHANISMS IS THEIMMUNOSUPPRESSION CAUSED BY THE DIRECTINFECTION AND FUNCTIONAL IMPAIRMENT OF IMMUNECELLS. HUMAN HERPES VIRUS TYPE 6 (HHV-6) IS ATYPICAL IMMUNOSUPPRESSIVE AGENT, ASSUGGESTED BY ITS TROPISM FOR BOTH CD4+ ANDCD8+ T CELLS, B CELLS,MONOCYTES/MACROPHAGES, MEGAKARYOCYTESAND NK CELLS. IN THIS STUDY THE PRODUCTION OFIL-10 AND IL-12 BY PERIPHERAL BLOODMONONUCLEAR CELLS (PBMC) WAS EVALUATEDDURING HHV-6 INFECTION "IN VITRO". OUR RESULTSDEMONSTRATE THAT HHV-6 UP-REGULATES IL-10PRODUCTION BY PBMC. FURTHERMORE, OUR DATASUGGEST THAT RHIFN GAMMA ADDITIONCOUNTERACTS THE EFFECT OF HHV-6 IN PROMOTINGIL-10 RELEASE. TO GAIN MORE INSIGHT INTO THEROLE OF IFN GAMMA, ANTI-IFN GAMMA MONOCLONALANTIBODIES WERE ADDED TO PBMC STIMULATEDWITH LPS. NEUTRALIZATION OF ENDOGENOUS IFNGAMMA UPREGULATED IL-10 RELEASE.FURTHERMORE, HHV-6 INFECTION INHIBITED IFNGAMMA RELEASE INDUCED BY LPS IN PBMC. NOBASAL PRODUCTION OF IL-12 WAS FOUND IN PBMC.MOREOVER, HHV-6 INFECTION DID NOT INDUCE IL-12RELEASE BY PBMC. ON THE CONTRARY, IL-12 WASDETECTED IN THE SUPERNATANTS OF PBMCTREATED WITH LPS WITH OR WITHOUT RHIFNGAMMA. IN THESE EXPERIMENTAL CONDITIONS THEFURTHER ADDITION OF HHV-6 MARKEDLY IMPAIREDIL-12 PRODUCTION. MOREOVER, THE


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NEUTRALIZATION OF IL-10 RESULTED IN ASIGNIFICANT UP-REGULATION OF IL-12. FINALLY OURDATA SUGGEST THAT THE IMMUNODYSREGULATIONINDUCED BY HHV-6 COULD BE ACCOUNTED FOR BY ASHIFT FROM A TH-1 TO A TH-2 TYPE CYTOKINEPROFILE.



Titolo

PRODUCTION OF TNF ALPHA AND INTERLEUKIN 6 BYDIFFERENTIATED U937 CELLS INFECTED WITHLEISHMANIA MAJOR.

Autori

ARENA A, CAPOZZA AB, DELFINO D, IANNELLO D.

Abstract

SEVERAL CYTOKINES PLAY A CRUCIAL ROLE IN THEDEFENSE OF THE HOST AGAINST PROTOZOABELONGING TO THE GENUS LEISHMANIA. HOWEVER,THE ROLE OF TUMOR NECROSIS FACTOR ALPHA (TNFALPHA) AND INTERLEUKIN 6 (IL-6) IN HUMANLEISHMANIASIS IS STILL CONTROVERSIAL. THE AIMOF THIS WORK WAS TO STUDY, IN AN "IN VITRO"MODEL, THE INTERACTIONS OF HUMAN PHAGOCYTESWITH L. MAJOR. THE U937 HUMAN MONOCYTIC CELLLINE, DIFFERENTIATED WITH PHORBOL MYRISTATEACETATE (PMA) OR A COMBINATION OF 1 ALPHA,25DIHYDROXYVITAMIN D3 (VD) AND RETINOIC ACID (RA),WAS USED IN ALL THE EXPERIMENTS. THE RATE OFINFECTION, AS WELL AS THE PRODUCTION OF TNFALPHA AND IL-6 BY CELLS UPON INFECTION WITHPROMASTIGOTES, WERE STUDIED. IT WAS FOUNDTHAT, DEPENDING ON THE AGENT USED FORDIFFERENTIATION, U937 CELLS PRODUCED


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DIFFERENT PATTERNS OF CYTOKINES. PMADIFFERENTIATED CELLS PRODUCED SIGNIFICANTLYMORE TNF ALPHA, BUT LESS IL-6 THAN CELLSDIFFERENTIATED WITH VD-RA. NO DIRECTRELATIONSHIP WAS FOUND BETWEEN THE ABILITYOF DIFFERENTIATED U937 CELLS TO RELEASE TNFALPHA OR IL-6 AND THEIR LEISHMANICIDAL ACTIVITY.



Titolo

TNF ALPHA AND IL-6 PRODUCTION BYDIFFERENTIATED U937 CELLS INFECTED WITHLEISHMANIA MAJOR.

Autori

IANNELLO D., CAPOZZA AB, ARENA A

Abstract


JOURNAL OF LEUKOCYTE BIOLOGY; P. 242 - 242, ISSN:0741-5400ANNO: 1996 - ISBN:

Titolo

INDUCTION OF INTERLEUKIN 1A IN MURINEMACROPHAGES INFECTED IN VITRO WITH DIFFERENTSPECIES AND STRAINS OF LEISHMANIA.


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Autori

DELFINO D., CHIOFALO M.S., RIGGIO G., ANGELICIM.C., GRAMICCIA M., GRADONI L., IANNELLO D.

Abstract

IT IS NOW GENERALLY AGREED THAT SEVERALCYTOKINES RELEASED BY IMMUNOCOMPETENTCELLS SUCH AS MACROPHAGES PLAY A CRUCIALROLE IN THE OUTCOME OF INFECTIONS CAUSED BYPROTOZOA BELONGING TO THE GENUS LEISHMANIA.IN PARTICULAR, TUMOR NECROSIS FACTOR (TNF)INDUCTION DURING THE COURSE OF CUTANEOUSLEISHMANIASIS HAS BEEN RELATED TO RESISTANCETO L. MAJOR INFECTION IN MICE. HOWEVER, THEROLE PLAYED BY INTERLEUKIN 1 (IL-1) IN THE HOSTRESPONSE TO LEISHMANIASIS HAS YET TO BECOMPLETELY ELUCIDATED. THE AIM OF THIS WORKWAS TO STUDY WHETHER DIFFERENT SPECIES ANDSTRAINS OF LEISHMANIA COULD INDUCE IL-1 ALPHAIN MURINE MACROPHAGES IN VITRO. RESIDENTPERITONEAL MACROPHAGES OF BALB/C ANDC3H/HEN MICE WERE INFECTED WITH L. DONOVANI, L.MAJOR, OR DIFFERENT STRAINS OF L. INFANTUM. ITWAS FOUND THAT L. DONOVANI DID NOT INDUCE IL-1ALPHA IN MACROPHAGES FROM EITHER MICESTRAIN. INFECTION WITH L. MAJOR OR WITH THREEOUT OF SIX STRAINS OF L. INFANTUM INDUCEDCONSISTENT AMOUNTS OF IL-1 ALPHA, BUT ONLY INMACROPHAGES FROM GENETICALLY RESISTANTC3H/HEN MICE. NO RELATIONSHIP WAS FOUNDBETWEEN THE RATE OF INFECTION OFMACROPHAGES AND THE AMOUNT OF IL-1 ALPHADETECTED IN THE SUPERNATANTS OF INFECTEDMACROPHAGES. DATA OBTAINED CONFIRM THAT THERELEASE OF IL-1 ALPHA BY MURINE MACROPHAGESINFECTED IN VITRO WITH LEISHMANIA ISINFLUENCED BY THE GENETIC BACKGROUND OF THECELLS AS WELL AS BY THE PARASITE SPECIES.


MICROBIAL PATHOGENESIS, VOL. 18; P. 73-80., ISSN:


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0882-4010ANNO: 1995 - ISBN:

Titolo

INDUCTION OF TUMOR-NECROSIS-FACTOR-ALPHA BYLEISHMANIA-INFANTUM IN MURINE MACROPHAGESFROM DIFFERENT INBRED MICE STRAINS.

Autori

CHIOFALO MS, DELFINO D, MANCUSO G, LATASSA E,MASTROENI P, IANNELLO D.

Abstract

THE PRESENT STUDY WAS UNDERTAKEN TODETERMINE WHETHER THE VISCEROTROPICSPECIES, LEISHMANIA INFANTUM, ENDEMIC IN ITALY,COULD INDUCE TUMOR NECROSIS FACTOR ALPHA(TNF ALPHA) IN MURINE MACROPHAGES.GENETICALLY SUSCEPTIBLE (LSHS) AND RESISTANT(LSHR) MICE WERE USED IN THE ATTEMPT TOCORRELATE TNF ALPHA PRODUCTION WITH THEABILITY TO CONTROL PARASITE GROWTH ANDREPLICATION. RESIDENT PERITONEALMACROPHAGES OF C3H/HEN, DBA/2, CBA (LSHR),C57BL/10 AND BALB/C (LSHS) MICE WERE INFECTEDIN VITRO WITH PROMASTIGOTES AT A PARASITE TOCELL RATIO OF 8:1. NO SIGNIFICANT DIFFERENCES INTHE PERCENTAGES OF INFECTED PERITONEALCELLS OF LSHS VERSUS LSHR MICE WEREOBSERVED UNTIL 72 H OF IN VITRO CULTURE. ON THECONTRARY, KUPFFER CELLS FROM LSHR MICEINHIBITED LEISHMANIA REPLICATION. PERITONEALMACROPHAGES OF RESISTANT MICE PRODUCEDSIGNIFICANTLY HIGHER AMOUNTS OF TNF ALPHA ASCOMPARED TO SUSCEPTIBLE MICE. TNF ALPHAPRODUCTION OF BOTH RESISTANT ANDSUSCEPTIBLE MICE PEAKED AT ABOUT 5 H AFTERTHE CHALLENGE WITH THE PARASITE. NO TNF ALPHAWAS FOUND IN SUPERNATANTS OF INFECTEDKUPFFER CELLS FROM ALL THE STRAINS TESTED.THE ABILITY OF MACROPHAGES FROM SUSCEPTIBLEOR RESISTANT MICE STRAINS TO PRODUCE TNF


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ALPHA AFTER CHALLENGE WITH LEISHMANIAINFANTUM DOES NOT SEEM RELATED TO THEIRCAPACITY TO CONTROL PARASITE REPLICATION INVITRO.


MICROBIAL PATHOGENESIS, VOL. 12; P. 9 - 17, ISSN:0882-4010ANNO: 1992 - ISBN:

Titolo

AMINOGLYCOSIDES MODIFY THE INVITROMETACHROMATIC REACTION AND MURINEGENERALIZED SHWARTZMAN PHENOMENON INDUCEDBY SALMONELLA- MINNESOTA R595LIPOPOLYSACCHARIDE.

Autori

FOCA A, MATERA G, IANNELLO D., BERLINGHIERI MC,LIBERTO MC

Abstract

ENDOTOXIN-NEUTRALIZING ACTIVITY MAY BE ANIMPORTANT PROPERTY FOR ANTIBIOTICS TO BEUSED IN SEVERE SEPSIS. SEVERAL ANTIBIOTICS,BELONGING TO DIFFERENT CLASSES, WEREEVALUATED AS TO THEIR ENDOTOXIN-NEUTRALIZINGABILITY, USING THE INHIBITION OF AN IN VITROMETACHROMATIC ASSAY FORLIPOPOLYSACCHARIDES AND A MURINEGENERALIZED SHWARTZMAN REACTION MODEL.GENTAMICIN, AMIKACIN, AND SISOMICIN HAVE BEENFOUND TO SHARE SIGNIFICANT IN VITROANTIENDOTOXIN ACTIVITY AT ANANTIBIOTIC/ENDOTOXIN RATIO AS LOW AS 1.0/5 (BYWEIGHT) AND TO REDUCE THE MURINE GENERALIZEDSHWARTZMAN REACTION AT ANANTIBIOTIC/ENDOTOXIN RATIO OF 3.3/5.


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ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, VOL.35; P. 2161 - 2164, ISSN: 0066-4804ANNO: 1991 - ISBN:

Titolo

ROLE OF EXOGENOUS INTERFERONS ON INTRINSICANTIVIRAL ACTIVITY OF MACROPHAGES FROMPATIENTS AFFECTED BY NEOPLASIA.

Autori

MERENDINO RA, ARENA A, LIBERTO MC, MESITI M,CHILLEMI S, IANNELLO D., BONINA L

Abstract

MACROPHAGES DERIVED FROM IN VITRO CULTUREDMONOCYTES WERE INFECTED WITH HERPESSIMPLEX VIRUS TYPE 2. A MARKED IMPAIRMENT INTHE INTRINSIC ANTIVIRAL ACTIVITY WAS FOUND INMACROPHAGES OBTAINED FROM PATIENTS WITHBREAST CANCER OR MELANOMA. MOREOVER, THEANTIVIRAL ACTIVITY OF MACROPHAGES FROMHEALTHY DONORS, DIFFERENTIATED IN SERUM FROMPATIENTS WITH NEOPLASIA, WAS ALSO IMPAIRED.THE AIM OF THIS WORK WAS THE EVALUATION OFALPHA, BETA, GAMMA EXOGENOUS INTERFERON INRESTORING THE INTRINSIC ANTIVIRAL ACTIVITY OFMACROPHAGES FROM PATIENTS AFFECTED BYBREAST CANCER OR MELANOMA UNDER DIFFERENTCONDITIONS. PRETREATMENT OF MACROPHAGESWITH ALPHA, BETA INTERFERONS, BUT NOT GAMMAINTERFERON, RESTORED THEIR IMPAIRED INTRINSICANTIVIRAL ACTIVITY.



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JOURNAL OF CHEMOTHERAPY, VOL. 2; P. 116 - 122,ISSN: 1120-009XANNO: 1990 - ISBN:

Titolo

P40 MODULATION OF MACROPHAGES FROMDIFFERENT ANATOMICAL SITES.

Autori

IANNELLO D, ALTAVILLA D, COSTA GB, SEMINARA S,DELFINO D, MASTROENI P.

Abstract


J CHEMOTHER. 1989 JUL;1(4 SUPPL):435-7.ANNO: 1989 - ISBN:

Titolo

DIFFERENT EFFECTS OF BACTERIALLIPOPOLYSACCHARIDE ON SUPEROXIDE ANIONPRODUCTION BY MACROPHAGES FROM NORMAL ANDTUMOR-BEARING RATS.

Autori

ALTAVILLA D, BERLINGHIERI MC, SEMINARA S,IANNELLO D, FOCÀ A, MASTROENI P.

Abstract

BACTERIAL ENDOTOXINS ORLIPOPOLYSACCHARIDES (LPS) EXHIBIT A WIDE


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RANGE OF MODULATORY ACTIVITIES ONIMMUNOCOMPETENT CELLS. AMONG THE NUMEROUSEFFECTS OF LPS ON MACROPHAGES, ANENHANCEMENT OF SUPEROXIDE ANION (O2-)RELEASE HAS BEEN REPORTED. IN PREVIOUSSTUDIES CARRIED OUT ON TUMOR-BEARING RATS, ITWAS FOUND THAT SEVERAL FUNCTIONS OFPERITONEAL MACROPHAGES SUCH AS PHAGOCYTIC,MICROBICIDAL AND ANTIVIRAL ACTIVITIES WEREDEPRESSED. IN THIS PAPER WE EVALUATED THESPONTANEOUS OR PHORBOL MYRISTATE ACETATE(PMA)-INDUCED PRODUCTION OF SUPEROXIDE ANIONBY MACROPHAGES FROM TUMOR-BEARING RATSWITH RESPECT TO CONTROLS. MOREOVER, THEEFFECT OF IN VITRO PRIMING WITH LPS ON O2-PRODUCTION BY THE SAME CELLS WAS STUDIED. ITWAS FOUND THAT THE PATTERN OF SUPEROXIDERELEASE BY MACROPHAGES FROM TUMOR-BEARINGRATS IS SIGNIFICANTLY DIFFERENT FROMCONTROLS. PREINCUBATION OF MACROPHAGESFROM NORMAL RATS WITH LPS ENHANCED THESPONTANEOUS AND PMA-INDUCED PRODUCTION OFO2-. IN CONTRAST, THE SAME CONCENTRATIONS OFLPS DID NOT PRIME MACROPHAGES FROM TUMOR-BEARING RATS.


IMMUNOPHARMACOLOGY. 1989 MAR-APR;17(2):99-106.ANNO: 1989 - ISBN:

Titolo

IMPAIRMENT OF MACROPHAGE ANTIVIRAL ACTIVITYBY SOLUBLE TUMOR PRODUCTS. EFFECTS OFBACTERIAL IMMUNOMODULATORS.

Autori

BONINA L, ARENA A, LIBERTO MC, IANNELLO D,MERENDINO RA, COSTA GB, MASTROENI P.

Abstract


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THE ANTIVIRAL ACTIVITIES OF NORMAL RATPERITONEAL MACROPHAGES VERSUS HERPESSIMPLEX VIRUS TYPE 1 WERE INHIBITED BY SERAFROM TUMOR-BEARING RATS AND 3M KCL EXTRACTSOF TUMOR MASS. THE INHIBITORY ACTIVITY WASDEMONSTRATED ON THE EXTRINSIC AS WELL AS ONTHE INTRINSIC MACROPHAGE FUNCTIONS. SERAFROM CORYNEBACTERIUM PARVUM AND LISTERIAMONOCYTOGENES TREATED TUMOR BEARING RATSDID NOT INHIBIT THESE MACROPHAGES FUNCTIONS.FURTHERMORE THE 3 M KCL EXTRACTS FROM THETUMOR MASS OF THE ABOVE TREATED TBR SHOW ADECREASE IN THE CAPABILITY TO IMPAIR THESEMACROPHAGE FUNCTIONS. ON THE OTHER HAND,THE TREATMENT WITH THE ORAL POLYVALENTADJUVANT "BUCCALIN" WAS NOT ABLE TO RESTORETHE COMPROMISED ANTIVIRAL ACTIVITY IN TUMORBEARING RATS.


G BATTERIOL VIROL IMMUNOL. 1988 JAN-DEC;81(1-12):10-24. ERRATUM IN: G BATTERIOL VIROLIMMUNOL 1989 JAN-DEC;82(1-12):214.ANNO: 1988 - ISBN:

Titolo

THE ANTIVIRAL ACTIVITIES OF NORMAL RATPERITONEAL MACROPHAGES VERSUS HERPESSIMPLEX VIRUS TYPE 1 WERE INHIBITED BY SERAFROM TUMOR-BEARING RATS AND 3M KCL EXTRACTSOF TUMOR MASS. THE INHIBITORY ACTIVITY WASDEMONSTRATED ON THE EXTRINSIC AS WELL AS ON

Autori

MERENDINO RA, ARENA A, LIBERTO MC, IANNELLO D,BONINA L, MESITI M, MASTROENI P.

Abstract


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IT HAS BEEN REPEATEDLY REPORTED THAT SEVERALFUNCTIONS OF MONONUCLEAR CELLS ARE IMPAIREDIN PATIENTS AFFECTED BY NEOPLASIA. MOREOVER,INHIBITORY ACTIVITY OF SERUM AND TUMOREXTRACTS ON MACROPHAGES HAVE BEENDESCRIBED. IN A PREVIOUS STUDY, WE FOUND AMARKED IMPAIRMENT OF THE INTRINSIC ANTIVIRALACTIVITY OF MACROPHAGES DERIVED FROMMONOCYTES ISOLATED FROM PERIPHERAL BLOODOF PATIENTS WITH BREAST CARCINOMA ORMELANOMA COMPARED WITH THAT FROM BLOOD OFNORMAL SUBJECTS. THE AIM OF THE PRESENT WORKWAS TO STUDY WHETHER THIS IMPAIRMENT WASDUE TO CIRCULATING INHIBITORY FACTORS.MACROPHAGES WERE DIFFERENTIATED IN VITRO INSERA FROM PATIENTS WITH NEOPLASIA AND IN SERAFROM HEALTHY DONORS AND THEN CHALLENGEDWITH HERPES SIMPLEX VIRUS TYPE 2 (HSV-2).MACROPHAGES FROM NORMAL SUBJECTS,INCUBATED WITH SERA FROM PATIENTS, WERESIGNIFICANTLY IMPAIRED IN THEIR INTRINSICANTIVIRAL ACTIVITY. THESE RESULTS SUPPORT THEPOSSIBILITY THAT CIRCULATING INHIBITORYFACTORS INFLUENCE THE FUNCTIONALITY OFMONONUCLEAR PHAGOCYTES IN THE TUMOR-BEARING HOST.


CANCER DETECT PREV. 1988;12(1-6):73-80.ANNO: 1988 - ISBN:

Titolo

EVALUATION OF MACROPHAGE ANTIVIRAL ACTIVITYIN PATIENTS AFFECTED BY NEOPLASIA.

Autori

MERENDINO RA, IANNELLO D, ARENA A, BONINA L,GRECO V, MESITI M, CHILLEMI S, MASTROENI P.


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Abstract

THE INTRINSIC ANTIVIRAL ACTIVITY OFMACROPHAGES HAS BEEN STUDIED IN HEALTHYDONORS AND IN PATIENTS AFFECTED BY BREASTCANCER AND MELANOMA. IN VITRO DIFFERENTIATEDMACROPHAGES FROM BLOOD-DERIVED MONOCYTESWERE INFECTED WITH MEASLES VIRUS, HERPESSIMPLEX VIRUS TYPE 2 AND ADENOVIRUS 17. THECHALLENGE WAS CARRIED OUT WITH DIFFERENTMULTIPLICITIES OF INFECTION AND THE SYNTHESISOF VIRUS WAS TESTED BY EVALUATING THE SINGLECYCLE GROWTH CURVE IN 24 H. THE RESULTSOBTAINED SHOW THAT THE RESTRICTION OF VIRUSINFECTIVITY BY MACROPHAGES IS STRONGLYINFLUENCED BY THE MULTIPLICITY OF INFECTION.THIS WAS PARTICULARLY EVIDENT WITH THEADENOVIRUS 17. MOREOVER, MACROPHAGES FROMPATIENTS WITH MELANOMA AND BREAST CANCERSHOWED AN IMPAIRMENT OF THE INTRINSICANTIVIRAL ACTIVITY IN COMPARISON WITH NORMALSUBJECTS.


MED ONCOL TUMOR PHARMACOTHER. 1988;5(3):191-7.ANNO: 1988 - ISBN:

Titolo

SPONTANEOUS AND INDUCED INTERFERONPRODUCTION BY PERIPHERAL BLOOD LEUCOCYTESFROM CONTROL POPULATION AND PATIENTS WITHHERPES GENITALIS.

Autori

IANNELLO D, TAYLOR MW, FIFE KH, CHEN L.

Abstract


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THE SPONTANEOUS AND PHA INDUCED LEVELS OFINTERFERON WERE MEASURED IN PERIPHERALBLOOD LEUCOCYTE CULTURES OF TWENTY-EIGHTINDIVIDUALS DIAGNOSED AS POSITIVE FOR HERPESGENITALIS, AND IN A GROUP OF CONTROL SUBJECTS.AS REPORTED BY CUNNINGHAM AND MERIGAN [1983]FOR HERPES LABIALIS, LEUCOCYTES FROMINDIVIDUALS WITH HERPES GENITALIS PRODUCEDLOW LEVELS OF INTERFERON SPONTANEOUSLY;HOWEVER, SIMILAR RESULTS WERE FOUND FORINDIVIDUALS WITHIN THE CONTROL POPULATION. NOSTATISTICALLY SIGNIFICANT DIFFERENCE COULD BEFOUND FOR PHA INDUCED INTERFERON LEVELS,ANTIGEN INDUCED INTERFERON LEVELS, ORHELPER/SUPPRESSOR CELL RATIOS BETWEEN THEHERPES GENITALIS POPULATION AND CONTROLPOPULATION. OUR RESULTS INDICATE THATINTERFERON DOES NOT PLAY A MAJOR ROLE IN THELATENCY OR RECURRENCE OF HERPES GENITALIS.


J MED VIROL. 1987 MAY;22(1):25-34.ANNO: 1987 - ISBN:

Titolo

X-LINKAGE OF THE EARLY IN VITRO ALPHA/BETAINTERFERON RESPONSE OF MOUSE PERITONEALMACROPHAGES TO HERPES SIMPLEX VIRUS TYPE 2.

Autori

ELLERMANN-ERIKSEN S, LIBERTO MC, IANNELLO D,MOGENSEN SC.

Abstract

THE GENETICS OF THE EARLY INTERFERONRESPONSE OF MOUSE PERITONEAL CELLS TOINFECTION WITH HERPES SIMPLEX VIRUS TYPE 2(HSV-2) WAS STUDIED IN SUSCEPTIBLE BALB/C AND


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MORE RESISTANT C57BL/6 MICE AND IN RECIPROCALCROSSES BETWEEN THESE MICE. WASH-OUTS OFTHE PERITONEAL CAVITY OF NORMAL C57BL/6 MICECONTAINED SIGNIFICANTLY MORE CELLS THANWASH-OUTS FROM BALB/C MICE. THEREFORE,INTERFERON INDUCTION WITH HSV-2 WAS STUDIEDUNDER STANDARDIZED CONDITIONS IN VITRO.PERITONEAL CELLS REACTED TO HSV-2 INFECTIONBY INTERFERON PRODUCTION IN A VIRUS DOSE-DEPENDENT MANNER. INTERFERON WAS DETECTEDFIRST AFTER 2 H AND PEAKED AFTER 24 H. CELLSFROM C57BL/6 MICE OF EACH SEX PRODUCEDSIGNIFICANTLY MORE EARLY INTERFERON THANCELLS FROM BALB/C MICE, AND CELLS FROM FEMALEBALB/C MICE PRODUCED MORE INTERFERON THANCELLS FROM MALES. THIS DIFFERENCE WAS NOTSEEN WITH C57BL/6 MICE. CULTURES OF HIGHLYPURIFIED ADHERENT CELLS YIELDEDAPPROXIMATELY 10 TIMES AS MUCH INTERFERON ASCULTURES OF NON-ADHERENT CELLS. SINCETREATMENT OF CELLS WITH CARBONYL IRON ANDSILICA SIGNIFICANTLY REDUCED THE AMOUNT OFINTERFERON PRODUCED, WHEREAS 2000 RAD OFIRRADIATION HAD NO OBVIOUS EFFECT, IT ISCONCLUDED THAT THE MAIN INTERFERON-PRODUCING CELL IN THE PERITONEAL CAVITY OFMICE IN RESPONSE TO HSV-2 IS OF THEMONOCYTE/MACROPHAGE LINEAGE. INTERFERONPRODUCTION IN PERITONEAL CELLS WAS FOUND TOBE QUANTITATIVELY INFLUENCED BY X-LINKED LOCIIN THAT CELLS FROM MALE (BALB/C FEMALE X C57MALE) F1 MICE, WHICH INHERIT THE X CHROMOSOMEFROM THE LOW-RESPONDING BALB/C FEMALES,PRODUCED SIGNIFICANTLY LOWER AMOUNTS OFINTERFERON THAN CELLS FROM THE OTHER THREEF1 GENERATION GENOTYPES. ALL INTERFERONSWERE CHARACTERIZED AS ALPHA/BETAINTERFERON. IT IS SUGGESTED THAT THE EARLYPRODUCTION OF ALPHA/BETA INTERFERON INRESPONSE TO HSV-2 IS INFLUENCED BY X-LINKEDLOCI, WHICH MIGHT BE INVOLVED IN SEX-LINKEDDIFFERENCES IN RESISTANCE TO HUMANHERPESVIRUSES.


J GEN VIROL. 1986 JUN;67 ( PT 6):1025-33.


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ANNO: 1986 - ISBN:

Titolo

THE RESTORATION OF IMPAIRED MACROPHAGEFUNCTIONS USING AS IMMUNOMODULATOR THECORYNEBACTERIUM GRANULOSUM-DERIVED P40FRACTION.

Autori

MASTROENI P, BIZZINI B, BONINA L, IANNELLO D,MERENDINO RA, DELFINO D, BERLINGHIERI MC,LEONARDI MS, ARENA A, LIBERTO MC, ET AL.

Abstract

MANY MICROORGANISMS AND COMPOUNDS OFMICROBIAL ORIGIN EXHIBIT IMMUNOMODULATORYACTIVITIES AND HAVE BEEN EXTENSIVELY USED INIMMUNOTHERAPY OF EXPERIMENTAL ANIMALTUMORS AND IN PATIENTS WITH NEOPLASIA. IN THISPAPER WE DESCRIBE THE EFFECT OF THE C.GRANULOSUM-DERIVED P40 FRACTION ON THEGROWTH AND METASTATIZATION OF THETRANSPLANTABLE EPITHELIOMA T8 OF GUÈRIN.MOREOVER, WE EVALUATED THE EFFECT OF P40TREATMENT ON SEVERAL DEPRESSED MACROPHAGEFUNCTIONS OF TUMOR-BEARING RATS. INPARTICULAR, THE PHAGOCYTIC AND CHEMOTACTICACTIVITIES OF SUCH CELLS WERE STUDIED, ASWELL AS THE ANTIVIRAL INTRINSIC AND EXTRINSICACTIVITIES AGAINST HSV-1 AND THE ANTI-TOXOPLASMA GONDII ACTIVITY. ALL THESEFUNCTIONS WERE DEPRESSED IN UNTREATEDTUMOR-BEARING RATS. ADMINISTRATION OF ASINGLE INTRAVENOUS INJECTION OF P40 FRACTIONLED TO THE RESTORATION OF ALL DEPRESSEDMACROPHAGE ACTIVITIES TO NORMAL VALUES. INPARTICULAR, THE POSSIBILITY OF RESTORING THEANTIMICROBIAL ACTIVITY OF MACROPHAGES FROMTUMOR-BEARING RATS BY TREATMENT WITH THISIMMUNOMODULATOR IS OF GREAT CONCERN WHENONE CONSIDERS THE INCREASING INCIDENCE OFOPPORTUNISTIC INFECTIONS IN


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IMMUNOCOMPROMISED HOSTS. RESULTS AREDISCUSSED IN TERMS OF BOTH THE POSSIBLEMECHANISM OF ACTION OF P40 AND OF ITSPOSSIBLE TARGET CELLS.


IMMUNOPHARMACOLOGY. 1985 AUG;10(1):27-34.ANNO: 1985 - ISBN:

Titolo

EFFECT OF ORAL ADMINISTRATION OF DIFFERENTCOMBINATIONS OF KILLED BACTERIA ON SOMEDEPRESSED MACROPHAGE FUNCTIONS IN TUMOR-BEARING RATS.

Autori

IANNELLO D, BONINA L, DELFINO D, BERLINGHIERIMC, MASTROENI P.

Abstract

N THE PRESENT STUDY, WE COMPARED THE ABILITYOF DIFFERENT BACTERIAL SPECIES ADMINISTEREDORALLY IN VARIOUS COMBINATIONS TO RESTORESOME DEPRESSED PERITONEAL MACROPHAGEFUNCTIONS IN TUMOR-BEARING RATS.PHAGOCYTOSIS, KILLING OF CANDIDA ALBICANS ANDCHEMOTACTIC RESPONSE OF RESIDENTPERITONEAL CELLS FROM TREATED TUMOR-BEARING RATS WERE INFLUENCED BY DIFFERENTASSOCIATIONS OF BACTERIA. IN PARTICULAR, WHENSTAPHYLOCOCCUS AUREUS WAS ADMINISTEREDTOGETHER WITH OTHER BACTERIAL SPECIES, THEPHAGOCYTIC ACTIVITY OF PERITONEAL CELLS WASRESTORED TO NORMAL VALUES, ANDINTRACELLULAR KILLING OF C. ALBICANS WASENHANCED. THE RESULTS ARE DISCUSSED INRELATION TO THE POSSIBLE INFLUENCE OFMUCOSAL BACTERIAL FLORA ON THE LEVEL OFACTIVATION OF PERITONEAL MACROPHAGES. THE


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POSSIBILITY THAT BACTERIAL SPECIES CANINFLUENCE IN VARIOUS WAYS IMMUNOCOMPETENTCELLS IN RELATION TO THE DIFFERENT CHEMICALCOMPOSITION OF SOME COMMON STRUCTURES ISALSO DISCUSSED.


IMMUNOPHARMACOLOGY. 1985 JUN;9(3):181-7.ANNO: 1985 - ISBN:

Titolo

EVALUATION OF CORYNEBACTERIUM GRANULOSUMDERIVED P40 FRACTION EFFECTS ON MACROPHAGEANTI-HERPES SIMPLEX VIRUS TYPE 1 FUNCTIONS.

Autori

IANNELLO D, BONINA L, MERENDINO RA, ARENA A,MASTROENI P, BIZZINI B.

Abstract


ANTIVIRAL RES. 1985;SUPPL 1:167-71. NO ABSTRACTAVAILABLE.ANNO: 1985 - ISBN:

Titolo

PRODUCTION OF LYMPHOKINES AND INTERFERON BYIMMUNE CELLS INVOLVED IN RECOVERY OF MICEFROM HERPES SIMPLEX VIRUS TYPE 2 HEPATITIS.

Autori


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IANNELLO D, MOGENSEN SC.

Abstract

ADOPTIVE TRANSFER OF SPLEEN CELLS FROM MICE4 DAYS AFTER INFECTION WITH HERPES SIMPLEXVIRUS TYPE 2 (HSV-2) REDUCED THE VIRUS TITER INTHE LIVER OF RECIPIENT MICE INFECTED 24 HBEFORE TRANSFER. MACROPHAGE CHEMOTACTICFACTOR (CF) AND MACROPHAGE MIGRATIONINHIBITION FACTOR (MIF) WERE PRODUCED BY DAY 3OF INFECTION IN SPLEEN CELL CULTURESSTIMULATED WITH HSV-2, BUT NOT WITH CONTROLANTIGEN, I.E. 1 DAY BEFORE THE CELLS ARE ACTIVEIN ADOPTIVE TRANSFER. INTERFERON WASPRODUCED IN CULTURES ESTABLISHEDTHROUGHOUT THE INFECTION BUT NOT IN NORMALSPLEEN CELLS. FROM DAYS 1 TO 5 OF INFECTIONINTERFERON WAS PRODUCED IRRESPECTIVE OF INVITRO RESTIMULATION, ALTHOUGH THE HIGHESTAMOUNTS WERE ALWAYS PRODUCED AFTERSTIMULATION WITH THE SPECIFIC ANTIGEN. SPLEENCELLS FROM MICE INFECTED FOR 6 DAYS PRODUCEDINTERFERON ONLY WHEN STIMULATED WITH HSV-2.THE CELLS FROM 6-DAY-IMMUNE MICE ACTIVE INADOPTIVE TRANSFER AND CF AND MIF PRODUCTIONWERE FOUND TO BE THY 1+, IG- AND LYT2-. BOTH THY1+ AND PLASTIC ADHERENT CELLS WERENECESSARY FOR INTERFERON PRODUCTION,WHEREAS IG+ AND LYT2+ CELLS DID NOT PRODUCEINTERFERON. THE INTERFERON WAS ACID STABLEAND NEUTRALIZED BY ANTISERUM AGAINSTALPHA/BETA-INTERFERON AND THUS HAS THECHARACTERISTICS OF ALPHA-INTERFERON. THEDATA INDICATE THAT A DELAYED TYPEHYPERSENSITIVITY REACTION WITH LYMPHOKINE-INDUCED MACROPHAGE RECRUITMENT INTOINFECTIOUS FOCI MAY BE A CENTRAL FEATURE OFTHE RECOVERY PROCESS IN HSV-2-INDUCEDHEPATITIS. A POSSIBLE ROLE OF INTERFERONPRODUCED BY THE ACCUMULATED CELLS NEEDSFURTHER INVESTIGATION.



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IMMUNOBIOLOGY. 1985 MAY;169(4):412-23.ANNO: 1985 - ISBN:

Titolo

EFFECT OF ORAL ADMINISTRATION OF A VARIETY OFBACTERIA ON DEPRESSED MACROPHAGEFUNCTIONS IN TUMOUR-BEARING RATS.

Autori

IANNELLO D, BONINA L, DELFINO D, BERLINGHIERIMC, GISMONDO MR, MASTROENI P.

Abstract

IN CONSIDERATION OF THE WELL DOCUMENTEDINFLUENCE OF NORMAL MICROBIAL FLORA ON THELEVEL OF ACTIVATION OF MACROPHAGES, WEEVALUATED THE EFFECTS OF ORAL ADMINISTRATIONOF BACTERIA ON RATS WITH DEPRESSEDMACROPHAGE FUNCTIONS. AN ORAL, KILLEDPOLYVALENT VACCINE (DIPLOCOCCUS PNEUMONIAETYPES I, II AND III, STREPTOCOCCUS HAEMOLYTICUS,STAPHYLOCOCCUS AUREUS AND HAEMOPHILUSINFLUENZAE), THE LIVE LYOPHILIZEDSTREPTOCOCCUS FAECIUM AND SPORES OFBACILLUS SUBTILIS, RESPECTIVELY, WEREADMINISTERED ORALLY TO IMMUNO-DEPRESSEDRATS. RESULTS DEMONSTRATE THE RESTORATIONOF PHAGOCYTOSIS, INTRACELLULAR KILLING ANDTHE CHEMOTACTIC ACTIVITY OF MACROPHAGES.THESE EXPERIMENTAL OBSERVATIONS SUGGESTTHAT BACTERIAL FLORA ASSOCIATED WITHMUCOSAE CAN INFLUENCE THE LEVEL OFACTIVATION OF PERITONEAL MACROPHAGES.


ANN IMMUNOL (PARIS). 1984 MAY-JUN;135C(3):345-52.ANNO: 1984 - ISBN:


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Titolo

INHIBITION OF NORMAL RAT MACROPHAGEFUNCTIONS BY SOLUBLE TUMOR PRODUCTS. EFFECTOF SYSTEMIC TREATMENT WITH BACTERIALIMMUNOMODULATORS.

Autori

IANNELLO D, BONINA L, DELFINO D, BERLINGHIERIMC, MASTROENI P.

Abstract

THE PHAGOCYTIC AND CHEMOTACTIC ACTIVITIES OFNORMAL RAT PERITONEAL MACROPHAGES WEREINHIBITED BY SERA FROM TUMOR-BEARING RATS(TBR) AND 3 M KCL EXTRACTS OF TUMOR MASS.HOWEVER, SERA FROM CORYNEBACTERIUMPARVUM- OR LISTERIA MONOCYTOGENES-TREATEDTBR DID NOT INHIBIT PHAGOCYTOSIS. ON THE OTHERHAND, SERA FROM C. PARVUM-TREATED, BUT NOTFROM L. MONOCYTOGENES-TREATED TBR STILLINHIBITED THE CHEMOTACTIC RESPONSE OF THENORMAL MACROPHAGES. FURTHERMORE, 3 M KCLEXTRACTS OF TUMORS FROM C. PARVUM-TREATEDTBR DID NOT INHIBIT PHAGOCYTOSIS ANDCHEMOTACTIC RESPONSE OF THE SAME CELLS.SIMILAR RESULTS WERE OBTAINED WITH EXTRACTSOF TUMOR MASSES FROM L. MONOCYTOGENES-TREATED RATS. IT IS SUGGESTED THAT TREATMENTWITH BACTERIAL IMMUNOMODULATORS CANINFLUENCE THE RELEASE FROM NEOPLASTIC CELLSOF SOLUBLE PRODUCTS INFLUENCING NORMALMACROPHAGE FUNCTIONS.


CANCER IMMUNOL IMMUNOTHER. 1984;17(1):38-41.ANNO: 1984 - ISBN:

Titolo


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PHAGOCYTIC AND INTRACELLULAR KILLING ACTIVITYOF RAT MACROPHAGES: CRITICAL ANALYSIS OF 2DIFFERENT EVALUATION METHODS

Autori

IANNELLO D, BONINA L, CARBONE M.

Abstract

TWO DIFFERENT EVALUATION METHODS OF THE "INVITRO" BACTERIAL KILLING ACTIVITY OFMACROPHAGES WERE COMPARED; THE FORMERBASED ON THE DETERMINATION OF THE NUMBER OFVIABLE MICROORGANISMS IN THE SUPERNATANT OFMACROPHAGE CULTURES BY A MICROBIOLOGICALPLATE METHOD; THE LATTER BASED ON THEEVIDENTIATION OF INTRACELLULAR KILLING BYDIFFERENTIAL STAINING OF LIVING AND KILLEDMICROORGANISMS WITH ACRIDINE ORANGE.PHAGOCYTIC AND MICROBICIDAL ACTIVITIES OFPERITONEAL CELLS WERE INVESTIGATED BY THETWO METHODS IN CONTROL RATS AND IN TUMOR-BEARING RATS. QUALITATIVE AND QUANTITATIVEDIFFERENCES IN THE KINETICS OF PHAGOCYTOSISAND MICROBIAL KILLING WERE EVIDENTIATED INMACROPHAGES FROM TUMOR-BEARING RATS.FURTHERMORE, BOTH METHODS PROVED TO BESUITABLE AND REPRODUCIBLE.


G BATTERIOL VIROL IMMUNOL. 1984 JAN-JUN;77(1-6):45-53.ANNO: 1984 - ISBN:

Titolo

TUMOR-DEPENDENT RESISTANCE OF RATPERITONEAL MACROPHAGES TO HERPES SIMPLEXVIRUS.


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Autori

BONINA L, IANNELLO D, MERENDINO R, ARENA A,MASTROENI P.

Abstract

BY THEIR POSITION AT SITES OF INITIAL INFECTIONAND THEIR WIDE DISTRIBUTION IN MAJOR ORGANSOF THE BODY, MACROPHAGES MAY BE DECISIVE INDETERMINING THE SUSCEPTIBILITY OR RESISTANCEOF THE HOST TO VIRUS INFECTION. MACROPHAGERESTRICTION OF VIRUS REPLICATION HAS BEENSHOWN TO BE CLOSELY RELATED TO VIRUS STRAINSOR VIRUS TYPES AND TO THE AGE OF THE INFECTEDHOST. WE REPORT THE EFFECTS OF THEDEVELOPMENT OF A SOLID TUMOR IN RATS ONINTRINSIC IN VITRO MACROPHAGE ACTIVITYAGAINST HERPES SIMPLEX VIRUS TYPE 1. THERESULTS OBTAINED WITH THE INFECTIOUS CENTERASSAYS AND THE ANALYSIS OF SINGLE-CYCLEGROWTH CURVES OF HERPES SIMPLEX VIRUS TYPE1 IN MACROPHAGES OBTAINED FROM NORMAL ANDTUMOR-BEARING RATS SHOWED A DEPRESSION OFANTIVIRAL ACTIVITY OF MACROPHAGES FROMTUMOR-BEARING RATS. THE POSSIBILITY OFIMMUNOMODULATION BY BACTERIAL ADJUVANTS ONTUMOR-BEARING RATS AND THE EFFECTS ON THEANTIVIRAL ACTIVITY OF PERITONEAL MACROPHAGESWERE FURTHERMORE DEMONSTRATED


INFECT IMMUN. 1983 FEB;39(2):575-9.ANNO: 1983 - ISBN:

Titolo

INFLUENCE OF SOME BACTERIAL COMPONENTS ONSOME MONONUCLEAR PHAGOCYTE SYSTEMFUNCTIONS.


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Autori

BONINA L, IANNELLO D, FOCA' A, MASTROENI P.

Abstract

THE EFFECTS OF LISTERIA MONOCYTOGENESTREATMENT ON SOME FUNCTIONS OF PERITONEALMACROPHAGES IN TUMOR-BEARING RATS WEREEVALUATED. IN PARTICULAR PHAGOCYTOSIS ANDINTRACELLULAR KILLING OF ESCHERICHIA COLI ANDCANDIDA ALBICANS AND CHEMOTACTIC RESPONSETO ACTIVATED SERUM WERE STUDIED IN UNTREATEDAND TREATED ANIMALS. DIFFERENT RESTORATIONOF MACROPHAGE FUNCTIONS, DEPRESSED INTUMOR-BEARING RATS, WAS OBTAINED BYTREATMENT WITH WHOLE FORMOLINATED L.MONOCYTOGENES OR ITS CRUDE EXTRACT.


G BATTERIOL VIROL IMMUNOL. 1982 JUL-DEC;75(7-12):199-210.ANNO: 1982 - ISBN:

Titolo

POLYCLONAL LYMPHOCYTE ACTIVATORS: ADJUVANTACTIVITY OF A CRUDE EXTRACT OFSTREPTOCOCCUS FAECIUM.

Autori

IANNELLO D, FOCA' A, BONINA L, MASTROENI P.

Abstract

A CRUDE EXTRACT OF STREPTOCOCCUS FAECIUM,PREPARED ACCORDING TO KRÄMER AND BRADIS


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METHOD, WAS UTILIZED TO FURTHER EXTEND OURPREVIOUS RESEARCH ON POLYCLONAL LYMPHOCYTEACTIVATORS. EXPERIMENTS WERE PERFORMEDUSING JERNE'S PFC METHOD ON 12 WEEK-OLDC3H/FEJ MICE IMMUNIZED WITH SHEEP RED BLOODCELLS TO STUDY THE ADJUVANT ACTIVITY OF THECRUDE EXTRACT BY VARYING ITS DOSE, TIMES OFADMINISTRATION, AND ADMINISTRATION ROUTE(INTRAPERITONEAL, INTRAVENOUS, SUBCUTANEOUS).THE EXTRACT, CONTAINING CELL MEMBRANES ANDCYTOPLASMIC FRACTION, WAS DEMONSTRATED TOHAVE ADJUVANT ACTIVITY WHICH DEPEND ON THEDOSE AND TIME OF ADMINISTRATION


G BATTERIOL VIROL IMMUNOL. 1982 JAN-JUN;75(1-6):9-15.ANNO: 1982 - ISBN:

Titolo

SELECTIVE DEPRESSION OF PHAGOCYTESINTRACELLULAR KILLING ACTIVITY.

Autori

IANNELLO D, BONINA L, DELFINO D.

Abstract


ADV EXP MED BIOL. 1982;141:199-206.ANNO: 1982 - ISBN:

Titolo


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EFFECT OF PREIMMUNIZATION WITH ITS CARRIER ONTHE CELLULAR SECRETION OF IGM ANTI-TNPANTIBODIES

Autori

IANNELLO D, MANCINI C, DORIA G.

Abstract

THE SECRETION RATE OF ANTI-TNP IGM ANTIBODIESHAS BEEN ASSESSED ON SPLEEN CELLS FROMUNPRIMED OR HRBC-PRIMED MICE FOLLOWINGIMMUNIZATION WITH TNP-HRBC. MEASUREMENT OFTHE ANTIBODIES SECRETION RATE WASPERFORMED BY THE HEMOLYTIC PLAQUE INHIBITIONASSAY. HRBC-PRIMING INDUCED GREATER NUMBERSOF ANTI-TNP PFC/SPLEEN BUT HAD NO EFFECT ONTHE ANTIBODY SECRETION RATE AND ON ITSHETEROGENEITY. IT IS NOTEWORTHY THAT BOTHSECRETION RATE AND ITS HETEROGENEITY WEREFOUND TO OSCILLATE WITH TIME AFTERIMMUNIZATION.


G BATTERIOL VIROL IMMUNOL. 1981 JUL-DEC;74(7-12):229-38.ANNO: 1981 - ISBN:

Titolo

LISTERIA MONOCYTOGENES ACTION ON VIRAL T8EPITHELIOMA GROWTH]

Autori

BONINA L, IANNELLO D, MERENDINO R, FERA MT.


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Abstract

EFFECTS OF LISTERIA MONOCYTOGENES ONNORMAL AND TUMOR-BEARING RATS WEREEVALUATED. INHIBITION OF T8 TUMOR AND ITSMETASTASES IN TUMOR-BEARING RATS TREATEDWITH L. MONOCYTOGENES WAS OBSERVED.MACROPHAGE PHAGOCYTE FUNCTIONS, DEPRESSEDIN TUMOR BEARING RATS, WAS RESTORED BY L.,MONOCYTOGENES. IN PARTICULAR, A DISSOCIATIONBETWEEN DIFFERENT MACROPHAGES FUNCTIONS INTUMOR-BEARING RATS WAS OBSERVED.


BOLL SOC ITAL BIOL SPER. 1980 DEC 15;56(23):2453-9.ANNO: 1980 - ISBN:

Titolo

[HISTOPATHOLOGICAL ASPECTS AND MACROPHAGEREACTIVITY IN RATS WITH GUERIN'S T8 VIRAL TUMORAFTER TREATMENT WITH CORYNEBACTERIUMPARVUM]

Autori

CARUSO R, BONINA L, CHIMICATA S, IANNELLO D,INFERRERA C, MASTROENI P.

Abstract


ARCH DE VECCHI ANAT PATOL. 1979 MAR;63(3):491-9.ANNO: 1979 - ISBN:

Titolo


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IMMUNOGENICITY OF CEFUROXIME (CEFEM 1) AND(CEFEM 2)]

Autori

FOCÀ A, IANNELLO D, BONINA L, FERA MT.

Abstract


G ITAL CHEMIOTER. 1979 JAN-DEC;26(1-2):257-61.ANNO: 1979 - ISBN:

CAPACITÀ ECOMPETENZE

PERSONALI

1985-1986: RESEARCH ASSOCIATE PRESSO IL DEPARTMENTOF BIOLOGY DELLA INDIANA UNIVERSITY, BLOOMINGTON,INDIANA, USA; HA COLLABORATO CON IL PROF. MILTONTAYLOR.

1983-1984: VISITING RESEARCHER PRESSO L'INSTITUTE OFMEDICAL MICROBIOLOGY DELL'UNIVERSITÀ DI AARHUS,DANIMARCA; HA COLLABORATO CON IL PROF. SORENMOGENSEN

VINCITRICE DI UNA BORSA DI STUDIO DELLA FONDAZIONEBONINO-PULEJO, 1978

PRIMA LINGUA

ITALIANO

ALTRE LINGUE


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INGLESE

Capacità di lettura

ECCELLENTE

Capacità di scrittura

ECCELLENTE

Capacità di espressioneorale

ECCELLENTE

TEDESCO


BUONO


BUONO


BUONO

FRANCESE


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BUONO


BUONO


ELEMENTARE

DANESE


BUONO


ELEMENTARE


BUONO

CAPACITÀ ECOMPETENZERELAZIONALI

LA CONOSCENZA E LA PRATICA DELLA LINGUA INGLESESONO STATE ACQUISITE, DOPO DUE ANNI DI FREQUENZAAL BRITISH COLLEGE DI MESSINA, DURANTE IL SOGGIORNO


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IN DANIMARCA E SUCCESSIVAMENTE NEGLI USA. DURANTEIL SOGGIORNO IN DANIMARCA, SI ERA ACQUISITA ANCHEUNA BUONA CAPACITA’ DI LETTURA ED ESPRESSIONE ORALEIN LINGUA DANESE, FREQUENTANDO I CORSI DI DANESE PERSTRANIERI ORGANIZZATI DALLO SPROG CENTRET NELLACITTA’ DI AAHRUS. ENTRAMBI I SOGGIORNI ALL’ESTEROHANNO PERMESSO DI VIVERE E LAVORARE IN AMBIENTEMULTICULTURALE, CON UN RIFLESSO ESTREMAMENTEPOSITIVO SULLA PROPRIA FORMAZIONE PERSONALE ECULTURALE, QUINDI NON RISTRETTA SOLO ALLA CAPACITA’LAVORATIVA


ORGANIZZATIVE

COORDINATORE DEL CORSO INTEGRATO DI MICROBIOLOGIADEL C.L. IN MEDICINA E CHIRURGIA.

RELATORE DI NUMEROSE TESI DI LAUREA E DISPECIALIZZAZIONE.

RESPONSABILE DI RICERCA DI UNA UNITÀ OPERATIVA-QUOTA 40% DI UN PROGETTO NAZIONALE1989/1996.

RESPONSABILE SCIENTIFICO UNITÀ DI RICERCA DELPROGRAMMA INTERUNIVERSITARIO COFINANZIATODAL MURST,1997-1999, PROT.N°. 9706247700_005

E' TITOLARE DI FONDI DI RICERCA PRA.


TECNICHE

DA AUTODIDATTA, USA ALCUNI PROGRAMMI DI SCRITTURAED ELABORAZIONE DI DATI.IN LABORATORIO,USA ALCUNI STRUMENTI E PROGRAMMI DIGESTIONE DEL LABORATORIO STESSO.

PATENTE OPATENTI


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PATENTE AUTO B


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