ewma 2014 - ep412 biocellulose/propolis nanocomposite membranas as promissory antimicrobial dressing
TRANSCRIPT
BIOCELLULOSE/PROPOLIS
NANOCOMPOSITE MEMBRANES AS PROMISSORY ANTIMICROBIAL DRESSING
(
Departamento de Química Geral e Inorgânica
Instituto de Química de Araraquara –
)Laboratório de Materiais Fotônicos LaMF
UNESP
Hernane da Silva Barud, , Adalberto Miguel de Araújo Júnior, , Andresa Piacezzi
Nascimento, , Edna Aparecida Barizon, Franciane Marquele-Oliveira, , Andresa Aparecida
Berretta, , Sidney José Lima Ribeiro 1 Institute of Chemistry, UNESP, CP 355, Araraquara-SP, 14801-970, Brazil
e-mail: [email protected]/[email protected]
Abstract
Antimicrobial nanocomposites based on Brazilian propolis ethanol extract solution and bacterial cellulose (BC) membranes have been
prepared using a simple and fully green approach. BC/propolis nanocomposites membranes are flexible, macroscopically homogeneous
and also present large amounts of propolis. SEM images and XRD analyses confirm the propolis incorporation on to BC membrane
nanostructure. The antibacterial activity in the BC/propolis demonstrated to be dose-dependent, supporting an interesting possibility,
the obtention of propolis based membranes at the desired concentrations, taking into consideration its application and its skin residence
time.
Biomedical Applications
Acknowledgements
Acetobacter xylinum
BC membrane and tube Nanocelluloses
Bacterial Cellulose
Substitute for skin Contact lenses
Scaffolds by Rapid
Prototype
BC-Fibroin Sponge
Scaffold
BC-Ag antimicrobial
dressing
BC-Ag antimicrobial
dressing Biological test
BC/Própolis Nanocomposites
(a)
(b)
(c)
(d)
BC BC-propolis
Never dried
BC-propolis membrane
10 20 30 40
Inte
nsi
ty (
a.u
.)
2 (degrees)
(BC)
(Prop)
P
r
o
p
o
l
i
s
4000 3500 3000 2500 2000 1500 1000 500
(e)
(d)
(c)
(b)
% T
ransm
ittance
Wavenumber (cm-1)
(a)
Bacterium Bacterial cellulose/propolis
A B C
BC without propolis 5.5 0.00 5.5 0.00 5.5 0.00
Staphylococcus aureus ATCC 25923 8 0.58 9 0.00 10 0.00
Staphylococcus aureus ATCC 43300 7 0.00 8 0.00 9 0.00
Staphylococcus epidermidis ATCC 14990 7 0.58 8 0.00 9 0.00
Table 2 - Mean diameter (in mm) of the zones of inhibition provided by biomembranes A, B and C. Values are mean SD (n=3).
SD: Standard Deviation
0
1
2
3
4
5
6
7
8
9
3 days 7 days 15 days
Wo
un
d s
ize
(mm
)
Periods
Control
BC
BC-propolis
50 100 150 200 250 300 350 4000
20
40
60
80
100
(c)
(b)(d)
(e)
% W
eig
ht
Temperatura (ºC)
(a)