NATASHA KAUR

AMARPREET KAUR

YAASHINI

SELVARAJAH

KRISNAVIMALA

KRISHUAN

NABILAH BINTI

YUSOF

NORIZA BINTI

ISHAK

AMIRAH BINTI

SHAARI

HILDA ANAK EMAK

AIN NAJWA BINTI

AMRAN

NURUL HIDAYAH

BINTI MANSOR

ONG SHWU CHYN

PUI WAN TING

WONG SIEW CHING

Five Mus musculus have been used to investigate the effect of

(ginseng jawa) to the liver, kidney and intestine within `

of experimental.

• Class of mammalian

• Easy to get

• Easy to handle-small in size

To know the effect of TalinumPaniculatum

size and

body weight of Mus musculus

Behaviour investigation of the

Mus musculus.

Talinum Paniculatum contains

vitamin c and

toxic

Tanin,

Saponin and

Flavoid.

So we carry out this experiment to see the side effects of Tanilum Paniculatum to the internal organs (intestine, kidney and liver) of Mus musculus.

Experiment applicable only to five Mus musculus.

only three leaves of TanilumPaniculatum used everyday.

Time limitation to only 20 days

DAVID FAIRCHILD,

COCONUT GROOVE,

FLORIDA

DR J.L HEID said that in his report :

In the green leaves of Talinum The Vitamin C

is about 160 mg per 100 gram

The Talinum show the phenomenally high content of vitamin C compared to guavas and sweet peppers.

It also probably has a considerable contents of carotenoid pigments.

Chemical content in Talinum paniculatumcomposed of

saponin,

flavanoid and

tanin.

Saponin - strengthen our body and

- improve blood circulation.

Saponin : can cause irritation in mucosa membrane, and can damage red blood cell through hemolysis

The root can be used to treat :

tiredness,

dizzy,

diarrhea,

aphrodisiacs and

pneumonia.

The leaves can be used to treat bump.

Also increase the sexual desire in male mencit.

5 mice (Mus musculus)

Bedding

3 ginseng jawa (Talinum paniculatum) leaves (0.5g) mix with 100 ml water

Mice food

Mice is weighed daily

Amount of water consumed are measured

Glove

Cotton and chloroform

Dissecting set

Dissecting tray and pins

Water

Beaker

Water was applied on the ventral side of mice

The kidney, small intestine and liver were dissected.

Fixation.

Dehydration.

Clearing.

Embedding.

Sectioning.

Coloring/ staining.

Fixation

Bouin’s fluid (<24 hours)

Bottles

Dehydration

Ethanol 70% (< 24 hours)

Ethanol 80%, 95%, 100% (< 1 hour)

Clearing

Toluene solution (< 24 hours)

Embedding (Paraffin processing) Paraffin solution (60C) (< 1 hour)

Paraffin solution (60C) (< 1 hour)

• (twice procedure)

Paraffin solution added in mold followed by the organ.

Tissue sample then was left for 5 days.

The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.

materials methods Diamond pen

Distilled water

Albumin

Slide and slide cover

Microtome

Brush

The slices was placed on the slide.

Few drops of distilled water/albumin are dropped on the slides.

Intestine-distilled water

Albumin - liver and kidney

Heating (30C)

Materials Methods Xylen 1 And 2

Alcohol+xylen (1:1)

Alcohol, Alcohol( 90%,80%,75%)

Distilled Water

Hematoksilin Solution

0.5 % Sodium Bicarbonate Solution

Running Water

Scott’s Solution

Eosin

Color stain used :

• Harris Hematoksilin- Eosin coloring.

• Soaking of slides-

• into 21 piece of jar containing different types of chemicals and liquid

• based on the duration given.

• Gluing: DPX / Canada balsam

• Heating of slides

• (37 C)

• 1 week

0

5

10

15

20

25

30

…

1 2 3 4 5 6 7 8 9

10

11

12

13

14

15

16

17

Result

1

2

3

4

5

Graph showing the increasing weight mass of the mice in 20 days

The weight of each mice

increase day by day

After taking Talinum paniculatum as their drink.

The Talinum paniculatum

rich with vitamin C

increases appetite

Gaining of body weight

Graph showing the amount of water and Talinum paniculatum consumed by the mice's

The goblet cell in this intestine secretes more mucous compared to the normal

intestine

40x magnification

Goblet cell

Wide spaces in between cells showing the sinusoid. This means that more enzymes

are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is

compact.

40 x magnification

The presence of vitamin C in Talinumpaniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.

prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.

The glomerular is tightly packed due to the toxic caused by the drug that is being

detoxify by the glomerular as compared to the normal kidney slide.

40x magnification

• act as rinsing agent to remove the blood from the organs, and its sterileNormal saline solution

• a fixing & preserving solution for the organsBouin’s solution-

• For dehydration processEthanol 70%,80%,95% & 100%

• used for the clearance purposeToluene

• to hold the organ tissues tightly during the sectioning process

paraffin

• Only the small intestine cell can withstand with the pressure.Distilled water

• to maintain the shape of the kidney and liver cell.Albumin

Effect of Talinum

paniculatum

Increase the appetite

Improve blood

circulation

Stimulate testosterone hormone to

enhance sexual desire

they will get attracted to mate becomes active and aggressive

Weight gain

Tannins precipitate proteins inhibits in some ruminant animals

for the absorption of nutrients from high-tannin grains

such as sorghum. Tannic acid does not affect absorption

of other trace minerals such as zinc, copper, and manganese in rats

Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.

The lethal dose of Talinum paniculatum around 6% is able to increase the animal's body weight.

Prove: the mice’s body weight increases drastically in that 20 days.

Damage of the weighing machine.

Damage of Microscope.

Thank you..