Propriedades das enzimas

Estudo de enzimaspara aplicao em Bio Fuel Cell

Peso molecular:149.5 kDapH timo :5.4Ponto isoeltrico : 6.91 Ativadores: Reagentes ativadores sulfidricosMercaptoetanolDitiotreitolCisteinaMetais pesados Agente quelanteInibidores:Metais pesados e reagentes -SH TioureaPurina e derivados de pirimidinaCloroetanol e fluoroetanolIodoacetamida1,10-fenantrolina8-hidroxquinolinaAnalogos de Beta-NADADHDesenvolvimento de BioFuel cellGDH - glutamato desidrogenase

4 2-oxoglutarato + NH3 + NADH lutamato + NAD + H2O (glutamato desidrogenase)Desenvolvimento de BioFuel cellOcorrncia NaturalGDH

GDH Esquema fuel cell ** ver mediador usado

Peso Molecular: 160,000 DaPonto Isoeltrico : 4.2pH timo : 5.5 Temperatura Otima : 40 C

Inhibitors: Ag+, Hg2+, Cu2+

GOXPeso Molecular : 34 kDaPonto Isoeltrico : 4.9pH timo : 9.0 Temperatura tima : 55Inibidores: Ag+ Hg2 +MalDHPeso Molecular : 40.000 Ponto isoelectrico : 7.2pH timo : 7.0 Temperatura otima: 40 C

HRPPeso Molecular = 62000 DaPonto Isoeltrico 2.6 7.6pH timo = 3.0 Temperatura timo a 58 C. laccase12

Characteristics of LDH from Beef Heart:Molecular Weight). 136,700

Specificity: The enzyme is specific for L(+)lactate. Meister (1950) reports it reduces several -keto and ,-diketo acids but at about one-tenth the rate of reduction of pyruvate.Inhibitors: LDH is quite stable. It is inactivated by iodide. Inhibition by p-mercuribenzoate is slow. See Schwert and Winer (1963); also Andersonet al. (1974) and Bloxhamet al. (1975).Activators: A number of organic compounds which stabilize the enzyme, such as dimethyl sulfoxide, ethanol, and methanol, are reported by Georgeet al. (1969). Diethylstilbestrol and several of its derivatives also stabilize the enzyme (Cohenet al. 1969).

LDH

TamanhoN sitiosInibidoresAtivadoresTemp.pHPIsubstrato

*Nafion pKa, fornecedor

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