programas e bibliografias - unicamp · aulas teóricas aulas práticas aulas de estudo dirigido...

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UNICAMP – Universidade Estadual de Campinas

DAC – Diretoria Acadêmica Rua Sérgio Buarque de Holanda, 251 - Cidade Universitária – Barão Geraldo – Campinas/SP – 13083-970

Diretoria de Registro e Gerenciamento Acadêmico – 00 55 19 3521 6662 www.dac.unicamp.br

Rubrica:

UNIVERSIDADE ESTADUAL DE CAMPINAS DIRETORIA ACADÊMICA

PROGRAMAS E BIBLIOGRAFIAS

DISCIPLINA NOME

BI005 Tópicos Avançados em Processos de Fabricação de biocombustíveis

Horas Semanais Aulas Teóricas Aulas Práticas Aulas de Estudo

Dirigido Horas em Sala de

Aula * 60 0 0 Nº semanas Carga horária total Créditos Frequência Aprovação 15 60 4 75 % Nota e frequência

Ementa: - EMENTA CONFORME CATALOGO 2017

Objetivos:

This aims are to give a basic and updated vision of bioethanol production processes and open up possibilities to recognize improvements for the ethanol production, based one applications of classical and molecular genetics to modify metabolism and construct modified yeasts.

Programa: Ethanol production is a complex biotechnological process that requires different areas of knowledges and expertise (Laluce et al., 2012). For example, the evaluation of the performance of a fermentation process in large industrial reactors should take into account various threats or risks that can compromise the process as a whole. The main risks are the raises in the production costs, decreases in product yields, rate of product formation and eventually cause the entering at stuck fermentation. However, such risks can be diagnosed, interpreted and minimized at the fermentation stage making use of existing knowledges about the microbiology of contaminants (bacteria and wild yeasts) and Saccharomyces cells, microbial metabolism at the level of enzymatic inhibition, along with classical genetics of yeasts and regulation of the genic expression. APROACHES OFFERED BY THE DICIPLINE- this course will be taught in as follows: Topic I, dedicated to the application of basic microbiology tools to indicate perturbations in processes and control of variations in the microbial population during fermentation, involving following subtopics: · Cellular structures and diversity of microbes; · Prokaryotes and eukaryotes relevant in the ethanol production process; · Gram positive and Gram negative bacteria; · Microbial resistance to stresses; · Population dynamics; · Natural and chemical antibacterial agents. Topic II- Dedicated to the use of genetics as instrument to understand the alterations in process parameters, isolation and selection of new yeasts, construction and uses of genetically improved yeasts, through the following subtopics: · Cell cycle division; · Genetic instability, · Mutations, · Theory of genetic evolution; · Methods based on molecular genetics (DNA sequencing)to differentiate between cells yeasts and contaminants Topic III- Dedicated to correlations between process conditions and the metabolic reactions directly involved with the ethanol production, as follows: · enzymes involved with hydrolysis and detoxification of raw materials; · oxidative reactions leading to the ethanol production (glycolysis); · metabolic by- products of the fermentation and effects on metabolism; · redox balance of the metabolism · re-oxidation of the energy transporters (coenzymes rich in energy) by the respiratory chain; Topic IV- dedicated to the production of the ethanol from sugarcane juices and molasses, which involves the following subtopics: · main steps of the process, which area harvesting and cleaning of the sugarcane, clarification, concentration; fermentation, and distillation;





Rubrica:



· raw materials and composition; · surface phenomena (cellular adhesions); · chemical and microbiological monitoring of processes; · use of differential media to identify contaminants and mutants ; · Strategies to improve efficiency, reduce energy and raw material consumption process management) Topic V- Dedicated to the production of the ethanol from sugarcane bagasse involving subtopics as follows: · lignocellulosic materials; · pretreatments; · enzyme cocktails to degrade lignocellulosic materials; · characteristics required for the ethanol producer organisms; · inhibition of ethanol the producer yeast by compounds generated by process conditions Bibliografia: Gallardo J. C., Souza C S., Cicarelli R. M, Oliveira K. F., Morais M. R., Laluce C. (2011). Enrichment of a continuous culture of Saccharomyces cerevisiae with the yeast Issatchenkia orientalis in the production of ethanol at increasing temperatures.Industrial Microbiology and Biotechnology 38(3):405-146. Laluce C., Schenberg A.C., Gallardo J.C., Coradello L.F., Pombeiro-Sponchiado S.R.(2012) Advances and developments in strategies to improve strains of Saccharomyces cerevisiae and processes to obtain the lignocellulosic ethanol-a review. Applied Biochemistry and Biotechnolology.166(8):1908-26. Laluce C.; Longo E.; Pombeiro-Sponchiado S. R.; Cilli E. M.; Gallardo, J.C.M.; Masiero M.O.C. A complexidade da produção do bioetanol em fermentações abertas de matéria primas industriais. In: Bioenergia, desenvolvimento e pesquisa (Lemos, E.C.M. e Stradiotto N., NRT, Org.), Produção de biocombustíveis (Pat I), Cultura Academia, pp.165-194, 2012. Miranda I. Masiero O.; Zamai, T.; Capella, M. and Laluce C. (2015). Improved pretreatments applied to the sugarcane bagasse and release of lignin and hemicellulose from the cellulose-enriched fractions by sulfuric acid hydrolysis Journal of Chemical Technology and Biotechnology DOI 10.1002/jctb.4601published online Souza, C S., Oliveira, K F., Trevisan, H. C.; Laluce, C. (2007). A strategy to compare yeast strains and improve cell survival in ethanol production process above 30°C. In Communicating current Research and Education Topics and Trends in Applied Microbiology, pp.410-417. FORMATEX; Verlag Wiley-VBC Publishing. Solomon, E., Martin, C., Martin, D. W.; Berg, L. R. (2015) Study Guide for Biology, 10th ed., Singapore: Cengage Learning, Publishing. Walker, Graeme M. (1988). Colonial yeast growth in yeasty physiology and biotechnology, pp 127-131. Chichester: John Wiley and Sons Publishing.

Critérios de Avaliação:

STRATEGY TO BE ADOPTED DURING CLASSES EVALUATION CRITERIA During classes slides will be presented and commented in English and whenever necessary in Brazilian Portuguese to stimulate learning and interest of the students. In addition, selected bibliographical material will be suggested to students as exercises for use of information given during classes, prepare revision works and seminars. The student learning assessment will be based on the following criteria: · Questionings of students during classes; · Preparation of reviews to be submitted for evaluation; · Seminars presented orally to colleagues attending the course

Observações: (período mais provável de oferecimento)





Rubrica:



ASSINATURAS: (Prof. Responsável, Coordenador do Programa, Coordenador CPG/FEA, Diretor)

Instruções: *Horas em sala - Ao total de horas de aulas teóricas ministradas (T) deverá obrigatoriamente ser associada carga horária em sala de aula.

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