Identificação de Identificação de levedurasleveduras

Taxonomia clássica

Extrato de malte

MacroscopiaSedimentoResíduoAnelIlhotaPelículaMicroscopiaForma e tamanhodas células

MicrocultivoAgar fubáTween 80Corn meal agar

MicroscopiaPseudomicélio,Micélio verdadeiro,BalistósporosClamidósporos

MicroscopiaProdução esporos sexuais:Ascos e ascósporos

Meio de acetatoGorodkowaYMÁgua de torneira

Outras provas:-Urease-Cápsula-Meio DBB

IDENTIFICAÇÃO GENÉRICA

AuxanogramaAssimilaçãoFontes C e N

ZimogramaFermentação Alcoólica de açúcares

Meios diferenciais-meio TTC-ágar molibdênio-ágar azul Tripan-meio de Nickerson-meio de CGB

Outras provas:-Crescimento 37ºC-Produção de compostos amilóides-tubo germinativo-resistência aCicloheximida-fenol oxidase

IDENTIFICAÇÃO ESPECÍFICA

The xylose-fermenting yeast, Pichia stipitis (A) during vegetative growth, (B) forming spores, and (C) under carbon limitation. (FPL photo-Tom Kuster)  

2006 Highlights

  

o How Well Do Arsenic-Free Preservatives Inhibit Copper-Tolerant Fungi?

o

Caulking with Care o Combustion Properties of an Exotic Annual Grass o Creosote Movement from Treated Wood Immersed in Fresh Water o Durable Wood, Naturally--Termite Resistanceo Essential Oils Inhibit Mold Spore Germination o Fire Resistance of Strucutral Composite Lumber Products o Bioprocessing for Ethanol Production and a Valuable Adhesive Coproduct o

Fuel Treatment Evaluator o Fuel Treatment Marketo Genome Sequencing o Wood Research from World War I to Iraqo Reusing Remediated Wood in Value-Added Products o Inspecting Historic Structures: Using the Web to Train Inspection Professionals o Productive Use of Thinnings in Dimension Lumber and Papero Treatability of Underutilized Wood Species o Enhancing Wood-Plastic Composites by Crosslinking Polymerso Accessible and Affordable Playground and Path Surfacing Now Commerically Available o Dimensional Warping of Wood-Based Composites

The xylose-fermenting yeast, Pichia stipitis (A) during vegetative growth, (B) forming spores, and (C) under carbon limitation. (FPL photo-Tom Kuster)  

2006 Highlights

  

o How Well Do Arsenic-Free Preservatives Inhibit Copper-Tolerant Fungi?

o

Caulking with Care o Combustion Properties of an Exotic Annual Grass o Creosote Movement from Treated Wood Immersed in Fresh Water o Durable Wood, Naturally--Termite Resistanceo Essential Oils Inhibit Mold Spore Germination o Fire Resistance of Strucutral Composite Lumber Products o Bioprocessing for Ethanol Production and a Valuable Adhesive Coproduct o

Fuel Treatment Evaluator o Fuel Treatment Marketo Genome Sequencing o Wood Research from World War I to Iraqo Reusing Remediated Wood in Value-Added Products o Inspecting Historic Structures: Using the Web to Train Inspection Professionals o Productive Use of Thinnings in Dimension Lumber and Papero Treatability of Underutilized Wood Species

o Enhancing Wood-Plastic Composites by Crosslinking Polymers

o Accessible and Affordable Playground and Path Surfacing Now Commerically Available

o Dimensional Warping of Wood-Based Composites

The xylose-fermenting yeast, Pichia stipitis (A) during vegetative growth, (B) forming spores, and (C) under carbon limitation. (FPL photo-Tom Kuster)

                                            

          

A. Haploid yeast cells buddingB. Haploid cells forming shmoos and zygotesC. Zygote budding off diploidD. Diploid buddingE. Diploid forming asci with ascospores; freed haploid spores

Teste do DBB. As linhagens CCA 181, 506 e 567 são DBB negativas, enquanto a CCA379 é DBB positiva (utilizada como controle positivo)

ESPOROSSEXUAIS

Produçãode pseudomicélio

Candida albicans - pseudomicélio

Carbono Nitrogênio

Lin

hage

m

Erit

ritol

S

acar

ose

Raf

inos

e G

alac

tose

X

ilose

M

elez

itose

A

rabi

nose

C

elob

iose

M

anito

l M

elib

iose

G

licos

e E

tano

l M

alto

se

Ac.

Lac

tico

Ac.

Citr

ico

Ram

nose

A

c. S

uccí

nico

In

osito

l L

isin

a

Etil

amin

a

Nitr

ato

005 - + + + + + + - - - + - + - - - - + - + - 006 - + + + + + + + + + + + + - + + + - + + - 007 - + - + + + + - - - + - + - - - - - - + - 008 - + + - - + + - - + + - + - - - - + - - - 012 - + + + + + + - - - + - + - - - + + + + - 022 - + - + - + - - + - + - - - - - - - - nd - 023 - + + + + + + - - + + - + - - - - + - - - 027 - + - + - + + - - - + - + - - - - - - nd - 035 - + - + + - + - + - + - + - - - - - - nd - 037 - + + - + + - - - + + - + - - - - + - - - 053 - + + + + + + + - - + - + - - + + + + + + 059 - + - - + + + + - - + - + - - - + + + + + 077 - + + + - + - + + - + + + - - - + + + + + 083 - + + + + + + - - - + - + - - - - + - - - 115 - + - + + + + - + - + + + - + - - + + + - 151 - + - - - - + - - - + - - - - - - - + - - 155 - + - + + + + + - - + + + - - + + + + + + 181 - + - + + + + + + - + + + - + - + + + + - 209 - + + + + + - - - + + - - - - - + - - - 373 - + - + + + + - + - + + + - - - + + - + - 385 - - - - - - + - - - + - + - - - - - - - - 397 - + - + - + + - - - + - - - - - + + - - - 447 - + - + + + + - + - + + + - + - + + + + - 467 - + - + + + + - + - + + + - + - + - + + + 471 - + - + + + + - + - + + + - + - + - + + - 506 - + - + + + + + + + + + + - + - + + + + - 540 - + - + nd + + - + - + + + - + - + + + + - 567 - + - + + + + - + - + + + - + - + + + + - 593 - + + + + + + - + - + + + - + - + + + + - 610 - + - - + + + + + - + + + - + - - + + + - 612 - + - + + + + + + - + + + - + - + + + + -

1 - (+) = positivo; (-) = negativo e (nd) = não determinado;

Carbono Nitrogênio

Lin

hage

m

Erit

ritol

Sac

aros

e R

afin

ose

Gal

acto

se X

ilose

Mel

ezito

se A

rabi

nose

Cel

obio

se M

anito

l M

elib

iose

Glic

ose

Eta

nol

Mal

tose

Ac.

Lac

tico

Ac.

Citr

ico

Ram

nose

Ac.

Suc

cíni

co In

osito

l L

isin

a

Etil

amin

a

Nitr

ato

005 - + + + + + + - - - + - + - - - - + - + - 006 - + + + + + + + + + + + + - + + + - + + - 007 - + - + + + + - - - + - + - - - - - - + - 008 - + + - - + + - - + + - + - - - - + - - - 012 - + + + + + + - - - + - + - - - + + + + - 022 - + - + - + - - + - + - - - - - - - - nd - 023 - + + + + + + - - + + - + - - - - + - - - 027 - + - + - + + - - - + - + - - - - - - nd - 035 - + - + + - + - + - + - + - - - - - - nd - 037 - + + - + + - - - + + - + - - - - + - - - 053 - + + + + + + + - - + - + - - + + + + + + 059 - + - - + + + + - - + - + - - - + + + + + 077 - + + + - + - + + - + + + - - - + + + + + 083 - + + + + + + - - - + - + - - - - + - - - 115 - + - + + + + - + - + + + - + - - + + + - 151 - + - - - - + - - - + - - - - - - - + - - 155 - + - + + + + + - - + + + - - + + + + + + 181 - + - + + + + + + - + + + - + - + + + + - 209 - + + + + + - - - + + - - - - - + - - - 373 - + - + + + + - + - + + + - - - + + - + - 385 - - - - - - + - - - + - + - - - - - - - - 397 - + - + - + + - - - + - - - - - + + - - - 447 - + - + + + + - + - + + + - + - + + + + - 467 - + - + + + + - + - + + + - + - + - + + + 471 - + - + + + + - + - + + + - + - + - + + - 506 - + - + + + + + + + + + + - + - + + + + - 540 - + - + nd + + - + - + + + - + - + + + + - 567 - + - + + + + - + - + + + - + - + + + + - 593 - + + + + + + - + - + + + - + - + + + + - 610 - + - - + + + + + - + + + - + - - + + + - 612 - + - + + + + + + - + + + - + - + + + + -

1 - (+) = positivo; (-) = negativo e (nd) = não determinado;

Classificação das leveduras Reino Fungi

Divisão Eumycota Subdivisão Ascomycotina Subdivisão Basidiomycotina Subdivisão Deuteromycotina

Chaves de identificação Kreger-van Rij, N.J.W. The yeasts. A

taxonomic study. Elsevier, 1984.

Barnett, J.A., Payne, R.W., Yarrow, D. Yeasts: characteristics and identification. Cambridge Univ. Press, 1990.