Higher Group ProbesClass Level ProbesClade Level ProbesGenus Level ProbesSpecies Level ProbesPHAEO01 Phaeocystis 76NCHLO01 Non-Chlorophyta 76EHUXL B+4 Emiliania huxleyi 76CRYPT03 Cryptophyta Clade III 76AOST28S A+5 Alexandrium ostenfeldii 76CLADE1 Chrysochromulina & Prymnesium Clade I 75CLADE2 Chrysochromulina Clade II 75SCOST A Skeletonema costatum 74BOLI03 Bolidophyceae Clade I & III (Pacific strains) 74DINO E-12 Dinophyceae (incl. Apicomplexa) 73PMINI04 Prorocentrum minimum 72PMICA02 Prorocentrum micans 72PLIMA01 Prorocentrum lima 72PLIMA02 Prorocentrum lima 72PLIMA03 Prorocentrum lima 72PELA01 Pelagophyceae 72HTRIQ01 Heterocapsa triquetra 72GMIKI01 Gymnodinium mikimotoi species complex 72CRYPT02 Cryptophyta Clade II 72CHLO02 Chlorophyta 72NA1 Alexandrium tamarense (North America) 72Neapel18S A Alexandrium tamarense (Mediterranean) 72AOST18S G+15 Alexandrium ostenfeldii 72AOST28S Dshift3 Alexandrium ostenfeldii 72EUK1209 Eukaryotes 71OLIGR02H OLI51050,26041 70BOLI01 Bolidophyceae 70PRYM01 Prymnesiophyta 69PMINI01 Prorocentrum minimum 69PMINI03 Prorocentrum minimum 69PMINI05 Prorocentrum minimum 69PRORO01 Prorocentrum Clade 69PRORO02 Prorocentrum Clade 69PGLOB01 Phaeocystis globosa 69PGLOB02 Phaeocystis globosa 69PENN01 Pennate diatoms 69MPUSI D Micromonas pusilla 69CRYPT46 Cryptophyta Clades IV & VI 69CRYPT05 Cryptophyta Clade V 69CRYPT04 Cryptophyta Clade IV 69CRYPT01 Cryptophyta Clade I 69BOLI02 Bolidophyceae 69ATWE03 Alexandrium tamarense (Western Europe) 69ATNA02 Alexandrium tamarense (North America) 69Neapel18S B Alexandrium tamarense (Mediterranean) 69Neapel18S F Alexandrium tamarense (Mediterranean) 69AOST18S D Alexandrium ostenfeldii 69ATAM01 Alexandrium 69CPOLY01 Chrysochromulina polylepis 69DINO B Dinophyceae (incl. Apicomplexa) 69PSNPUN A-12 PSEUDONITZSCHIA PUNGENS 68HTRIQ B-3 Heterocapsa triquetra 65

ATWE03 Alexandrium tamarense (Western Europe) 69ATNA02 Alexandrium tamarense (North America) 69Neapel18S B Alexandrium tamarense (Mediterranean) 69Neapel18S F Alexandrium tamarense (Mediterranean) 69AOST18S D Alexandrium ostenfeldii 69ATAM01 Alexandrium 69CPOLY01 Chrysochromulina polylepis 69DINO B Dinophyceae (incl. Apicomplexa) 69PSNPUN A-12 PSEUDONITZSCHIA PUNGENS 68HTRIQ B-3 Heterocapsa triquetra 65OLIGR02F OLI51050,26041 62PLIMA new Prorocentrum lima 60PRYPOARV01 PRYMNESIUM PARVUM 60OLIGR03 OLI51059,33 60PSNMUL A+4 PSEUDONITZSCHIA MULTISERIES 58PSEUDONITSCHIA PSEUDONITZSCHIA 58PRYM03 PRYMNESIOPHYCEAE 58PavloA01 Pavlovaceae 58OLIGR04 OLI51102,16029 58COCCO01 CCOLITHALES 58PSNAUS A-8 PSEUDONITZSCHIA AUSTRALIS 54Hetero01 Heterokonta 54OLIGR01 OLI51080,76,47,6010 53PRYMGL01A PRYMNESIUMPRYMGL02B PRYMNESIUMPMICA01 Prorocentrum micansPMICA03 Prorocentrum micansGMIKI02 Gymnodinium mikimotoi species complexGMIKI03 Gymnodinium mikimotoi species complexCHAE01 ChaetocerosCHAE02 Chaetoceros

EUK 1209

CHLO02

DINO B

PELA01

12.06.00 02.08.01 03.22.01

03.22.0112.06.00

Tetraselmis Alexandrium Eucampia Chrysochomulina

Bolidomonad Achnanthes Alteromonas

DNA

PCR

ControlsPelagomonad

BOLIDO03

PRYM02

Probe Progress

aac

u cc u

AG A

A

u ga

u

c u g g u

g a c c g

UC

GC

AU

A C

AGC

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AU

UG

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UA

AU U C

A

GA G U

A

U

AG

A

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G G U C GC

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GGG

GG

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CG

CA

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GG U

UC U G

A C A

AC A

AG

UC

AA

AG

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UCC

AC

G

AGGGAU

ACUAC

UC

UGGUGG

G

UU

AA

A

A

CUC G

A

GA A

C GA

AA CG

AAUUCU

UUAGGG

C AUAAA

CU

AU

U

UAACCUC

UUGGAG

GGC

AAG U G

ACC

CUGG

UG U

G

G

GC

GU

AA

CCG

CCA

CA

UA

GAU

A U U G

U G

A C G

AU U U C C C G

GA A G G G C A

U

CC

A C C

UC

CU

UA

AG

GA

GU

AG

UA

GAUUACG

UC

CC

UUA C

GU

UU

AA C

GC

AA

AU

C

UCAA A

GGAAG

AUCCAC

GC

UGCCCGCCACA C

UU

AGA

UU

AG

CC

UAUCAU

GGUAAG

AAACCU

UGGCCU

UCA

G

GGA

GUUGGUUGUGAG

AAC

UU

GACCAGCGUU

AGG

GCUC

C

U

U U

UGGGA

CCUUU

U

G

G

GA

UC

GU

GGAA

CA

U

g

U

a a g a c g u c c

U U C C g u a g g

aag

u

aacua

GG

UCGC

GCGCA

AGC

CUGCG

GC

A C A C

GU

UU

UC

U

U GA

UG

CA

A AC

G

A

GG

GAU

AGG

AG

CAAGU

G

UU

G

G

CA

UU

G

G

C

UUAA

GU

UUC

GG

G

ACUC

AAC

A

A

A A C UU

A GGUCC

CCAGG

G C

CC

G

UC

CC

GG

UG

UAG

CAGA

GG U

GG

AU

AC

CU

AUUGA

G

AG

A

UC

UU

CC

U

AU

U

G

CC

A A CGG

A

U

UU

UC

AU U G

A

AGG U G

G

G UG

GCC

AA

UU

UUA

A

AAUUAG

UUAAUC

GG

AU

UUC

A

G

GC

U U

U

C

AA

G

UU

A

UA

AC

UG

A

U

UA

UA

g

CG

U

G

A

GUUG

GG

CC

U

GC UGU

AUG CG

A

AA

C

A C

UUU

GU

C

A

UGG

UU

A CG

G G G A

C C A C GG

A G A

UAAC

GG

G

A

UC

CGU

CG

UGCAG

A

G

A G A U

C

U C U

C A A

GA

A

U

U

U C

A U

G CU

U

A G C

C A G

C G U G

C

GAG

A

CAUUUG U

AU

A A A

U

UC

G UC

UAA

UUGA

AAC

C A UU

A

G U A G U

AA C

C

GA

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GAU

CG

GGC

GUA

GCA

CA

UGCG

AACGG

CU

CGA

GA

A

A

UA

UA

UA

AU

A

A

CG

GG

A

GC

CUU

AA

G

AC

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AA

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AA

GCGUAA

GA

CA

AU

AA AU

G U C C

C A G G

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AGGA

CUUU

AA

GU

CA

GA

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CGGG

A

UC

GA

CUG

U

AU

UA U

A

UU

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AA

G

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AU

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GU

CA

UA G

UC

CU

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GC

UUGCG

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AG

G

AU

CG

C UAG

CG

AU

UA

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AA

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A

AGAA

A

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GUU

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UG

GG

G

AC

CA

UG

G

GA

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CUA

C

G

U C

G G

AUCA

CA

AUU

U

C G

G CG

UU

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G G A U G G U A

C C U A C C A UGGU

GUAU

UUU

GAU

GG

U

AUA

GUU

UG AU

GG

A

G

AUGG

G

UACCA

CA

U

AUU

C U

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UA

U

G

U

G

G

U

GG

C

UU

CG

UU

GG

GC

GG

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CAA

GC

A U

UG

A

UU

GG

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C

C

G

G

UC

C

AA

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U

CAU

GA

CU

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G

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CG

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UG

AU

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A A U U

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AGG

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UA

AA

UA U A

AC

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C C U G U U U

G C C G A U G A G G UA

A

U

U A A U A A G A U A

G U U G U U U A

G GA

UUU

G

U

G U U A C C U U

U UC U G G A A

UC

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U

A U G CUU

G A C GA

A

C G

G C GA

AA

U

CG

AA

G G

U

AGUUAG

U UG

AU

U

1

2

3

4

5

6

7

8

9

E10-1

11

12

13

14

16

17

19

20

21

E21-1

E21-5

E21-6

E21-7

E21-8

E21-9

22

24

CUAC

GAUG

CG

AA G

U

GA

A

AU

C

A

UA

UG

25

26

UGA

GU

U

C

U

27

28

29

30

31

32

33

34

35

36

37

38

3940 41

42

43

AG

A

CG

G

CU

AC

C UU

44

4546

47

48

AACAAU

UUA

A A U CCCU U

AU

CG

AG

GAU CA

AC

G

UG

CG

G

GC

C

UU

UA

U

C

A

C

A

U

A

U

G

C A

A G

C

U

U

UA

CU

A

UA

23A

UC

G A G

U UA

GGA

A

U

G

U

U

A

AG U U C

C

G

U

U

U

AAU

10

GU

A CC

A

U

UG U

C

G

U

C

A

U

U

C

U

G

G

A G A U C

G U A GC

U U

AA

U

UA

AU

A

U A C

C U U G

AUC G

GA U A

C

G

A

C

CC

GGGG

G

GC

C AC

UG

GA

GA

G AU

E21-2

C

CGG

AU

CC A

GG

AA

CA

15

C

Navicula diserta ex Willhelmshaven

U

G

CA

CUU

U

G G

A

C

U C U C

A

U

C

G

C

C

U

AU

GGG

G C

A

CC

CU

U

C

U

GG

G

A

U GA

U

U

UG

AUCGUG

UA

C

CA

AU

G

U

C

UA

AU

C

A

GC

U

G

G

C

U

CG

C

G

G

G

UUU

A

G

clade 02

Pela01 Prym01

Prym02

Clade 1

Penn 01

Ch.poly

Euk 1209

Dino B

NCHLO Chlo01

Chlo02

Pela02

P.salina

Storeatu

Rhinomon

Rhodomar

RhoM1480

Rhodoabb

Psulcata

Crypt.ph

Guillard

CCMP325_Geminige

Tamphiox

Chroosp2

Komma

ChrM1481

ChrM1318

ChrM1312

Hrufesce

Hbrunnes

Hviresce

CryM0739

CRYM117

CRypKo6

CrypM42

ChilomoM

CryM1094

Goniomon

Crypt.sp

G.wittro

C.parado

Cyanopty

G.nostoc

F_distic

Crcd.coh0.01 substitutions/site

Crcd.cohF_distic

CruciplaCalyptro

HelladspCalptr1Calypsph

HOlocospcoccopelCoccpel2Coccpel3

CalcidisUMsibv.fUmbilsibOolifrag

CCMP_300PlpseudoPl.gayra

PleucartPleurelo

Pleur.spPlscherf

PlplacolretjapOchrover

PleuroroHymglobo

HymcoronOchronep

ALgirrobSyrapulc

ChyslameDicrater

Iso.litoISOCHRYS

ReticuloCCMP625

Geph.ocnGephocn1Gephocn2Hux_AtlaehuxDWNEhuxSarE.huxleyehuxTXB

ol26041ol51050

PlatysimPlatsimJ

Prym.nemprymparvPRYMPATEPrymparv

Ch.polylCh.Pc200PrymcalaCh.chito

Ch.minorCh.kappa

Ch.hirtaCh.erici

Ch.herdlChry.herd

ChryhirtCh.fraga

Ch.breviImantoniImanJapCCMP1404

ChCM1204 ChryparkChrybrev

oli33_56ol51059

CH.SPin4CHryspin

OL16029oli51102

ol16108Ch.leadbCh.lead2

ol26017Ch.campuCh.cymbiCh.strob

Ch.simplCh.scuteCHyparJPCh.parva

Ch. acanCh.trondCh.tron2

Ch.rotalCh.spTH2

Ol16010oli26047ol51076

ol51080ol51004

Phgl1524PhxSF162phgb1528phglgmexPhglsurphglEAtlphgl141

PHPOU360phpo145

Phan161Phan157

Phan_144phan143Phan142

PHcordatPH.jaahnPHsp559

BOJO-NZ DiacronePavgyran

Pavlosp.PAVL1416

PavlovasPavl1394

PavsalinExamthem

0.005 substitutions/site

Tetr.theGymbreveGymaureoGymn.mik

GymcfmikGymnmikiGymgalaGyrosp1Gyrosp2Gymngala

PfiesterPfi-likeCrypbrodPfipis1i

PfipisciCachhallH.nieiH.ildefiHeteroca

Penta.tyPARMcl14PARMCL12Scrip.nu GymnpulGymno sp

GymnsangProrPanaThorheimPARMCL09

PeridiniProrEmarProrlimaProrLim2ProrAren

ProrMacuProrConcGloeovisProcmicaProrMexiProcminiProrMin2

ProrolabPARMcl48LEPIDODIGyrod.sp

Gym.cateGymnosp2Gymnosp3Gymnsp5PARMcl31PARMcl20Gymnfusc

DInoacumDinonorvdinoacutDinonor2Phalrotu

PARMcl11 OstreovaFragilidPyrocystPyrocystPyro.bah Alex.tam

Alextama Atam31-4AlexfundAlex.funAtamNa19AtamNa1Aletamar

ActME217AlexcohoAlexostfAlexoste

AlosK324AlosK287AlexminuAlexminuAminal3t

AlexmargALtay2TALtay4tALtay1T Gonyspi2 Gonyspi2Gony.spi

GonyspinCeratocCertenueCerfusc1Cerafus2Gymnbei1Gymnsimp

G.simpleGymnosimPolarellGymnvari

SyCS-161Symbo082SyJCUSG-SymbmicrSymbmeanSymbcorcSymbpiloSymbiodi

SymbspPARMcl57Noct.iluC_cohnii

Crypcoh2AmphcartAmphibelAmylooceAmoebophOLI02001PARMCl37

PerkspPerkmarPeridfolSarocymu

5 changes

ProteromBlastohoBlastosp

Cafe.roeHypochyt CCMP1144CMP1144NT_aquela

BotrydiuVaucheriXanthone

BotridioasterionAsterkarRhapbelgFragcrotFragvireSYneulnaFragisla

fragstriFrag.sppSyedrasp

ThalelegTnitschiAsterforDiatomaRhabdone

Staurcon Pinnular cfCaloneStriasp.

SurirelUndatellPsNpuMGPsNpuMCPsNpuMPPseNmultPsNpuPBPseNpungPsNausMBFragopsi

LyrellaGomparv2 Cymbcymb

EncyonemAnomoeneAchnlancCocconeiAmphopro

CampdiscAmpmont2Amphocap

Amphipro EUnotDGM RossiaNavsubm2Nav.min2

SellapupPhaeotriEunotiaBacpaxil

Achnasp1AchnabonPseudoGoPseuGomp

NaviculaPFOLSYMBPBALSYMBPleurospNitzCFfrN.apicul

CylclostCyclopho MicrotabGramtocnLamprisc

BID.P782CymatbelPapelegaeucampia Phaeoscechaetros

ChaetdidChaetospPleulaevOdontsinBelleroc

LithodesStreptamDitylbriSkel1009SkelsubsskelcoskSkelcosPSkelcosASkpseMED

SkelmenzSKelmen2ThalpseuThalspThalprof

ThaleccnThalrotuPlanksolCyclmenecyc menDetoncon

Thalfluv PoroglacLauderiaLeptodanLeptomin

StephbroAulacdisAulacambAulanyanAulasuba

AulislanAULASKV2AulabAB8AulabAB2MelovarsMelvar3

Probosci CoscradiActinoptActinoc2Actinocy

RhizosetRHizose1GuinflacRHSETGR2RhimbricGuindeli

COHISTRICorecriocoreinerFlag46SEFlag94SCFlag31SEPARALIADICTYOCH

RHIZOCHRPSEUDOPEAPEDINEPelasubvPelagosuCCMP1395PelagomdCCMP1145

Aureocanpar.cl7par.cl35Pela1410AureLaguPulvinar

SarcinomN.salinaNannsal2Nannosp1NanngranNannosp2Nannocul

MonodopsVischeriEustigmaPseuchar

Flag11SAFlag77SCO_danicaM.striatM_papill

S.espinoC_chromoChrysosp

GirausteF_disticSaccopolChordatoDesmarli

SporocomAlarmargMacrocys

ScytolomEctosiliHeterosi

ChattoneVacuolarL_gigantP_megaspA_bisexu

Developa TKRREUPRRE

Lbyr.minCFRRN16LAF008242AF008241

10 changes

Bolidomonads

Pelagophytes

Eustigmatophytes

Silicoflagellates

Diatoms

Array of four A. tamarense and one A. lusitanicum strain from different geographic regions hybridised with species- and strain-specific FITC-labelled

probes.

In situ Hybridisation

DINO01

AOST02AOST01

In situ Hybridisation of Alexandrium Species with Specific rRNA Probes

<15

<10

>70

>50

>40

<5

Dino01

Aost01

Atam01

Atna01

Station

9

Station

14

ProbesA. tamarense siteA. ostenfeldii site