FNI 2C EM 1
Transmission Electron Microscope
FNI 2C EM 2
TEM Image of E. Coli
FNI 2C EM 3
TEM Image of Mitochondria
FNI 2C EM 4
TEM Image of Computer Chip
FNI 2C EM 5
SEM Image compared to TEM Image
FNI 2C EM 6
Transmission Electron Microscope
http://www.matter.org.uk/tem/ http://em-outreach.ucsd.edu/web-course/toc.html http://www.amazon.com/Transmission-Electron-Microscopy-
Textbook-Materials/dp/038776500X/ref=sr_1_1?ie=UTF8&s=books&qid=1224191760&sr=8-1
FNI 2C EM 7
TEM Operation
FNI 2C EM 8
UW Eau Claire’s TEM
FNI 2C EM 9
UW Eau Claire’s TEM
FNI 2C EM 10
TEM Phosphor Screen
FNI 2C EM 11
TEM Control Panel
FNI 2C EM 12
TEM Grids
All TEM’s ever done have examined only .3 mm3 of material
FNI 2C EM 13
TEM Components
FNI 2C EM 14
TEM Sample Preparation and Theory of Operation Samples for TEM must be electron transparent. That means they
must be thin enough for electrons to pass through. It is very difficult to achieve this. After the sample has been polished very thin an ion mill is used to
perform the final stages. An ion mill uses an argon plasma to remove material from the
sample. High energy argon ions strike the sample and atoms of the sample
are knocked away. TEMs can be used to image atoms. Some scientists have combined TEM chambers with reactors so
they can watch chemical reactions in progress.
FNI 2C EM 15
FIB Sample Preparation for TEM
FNI 2C EM 16
Electron Microscope Vendors
JEOL http://www.jeol.com/ Hitachi http://www.hitachi-hitec-uk.com/ Zeiss www.smt.zeiss.com/nts FEI www.feicompany.com Camscan www.camscan-usa.com Elektronen-Optik www.eos-do.com Tescan www.tescan.com KLA/Tencor/Amray http://www.kla-tencor.com/
FNI 2C EM 17
Notes
Growth of carbon nanotubes inside environmental TEM.