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Biologia Genmica

2 Semestre, 2017

Recombinao Homloga e Stio-Especfica

Prof. Marcos Tlio

mtoliveria@fcav.unesp.br

Faculdade de Cincias Agrrias e Veterinrias de Jaboticabal

Instituto de Biocincias, Letras e Cincias Exatas de S.J.R.P.

Universidade Estadual Paulista Jlio de Mesquita Filho

mailto:mtoliveria@fcav.unesp.br

Aula Passada - Reparo de DNA

Recombinao

Recombinao para Reparo

Double-Strand

DNA Breaks

NHEJ

Recombinao

Homloga

Recombinao

Homloga

Recombinao

Homloga

Recombinao Homloga - Reparo

Sistema RecBCD de E. coli

Sistema RecBCD de E. coli

Invaso de ssDNA - RecA de E. coli

Invaso de ssDNA - RecA de E. coli

Yu et al., 2001. PNAS 98:841924

Invaso de ssDNA - RecA de E. coli

Invaso de ssDNA

- RecA de E. coli

Migrao de ssDNA - RuvAB de E. coli

Migrao de ssDNA - RuvAB de E. coli

https://www.sheffield.ac.uk/mbb/ruva/ruva-040

Resoluo - RuvC de E. coli

Recombinao

Meitica

Recombinao

Meitica

Troca de Sexo? S levedura mesmo...

Troca de Sexo? S levedura mesmo...

Recombinao Homloga - Resumo

Rad54, Rad5, BLM, WRN,

RECQ1, RECQ5, FANCM, etc. RecG

Embaralhando o Genoma...

Recombinao Stio-Especfica

Recombinao Stio-

Especfica

Serina e Tirosina Recombinases

Integrase do Bacterifago Lambda

Hin

Recombinase

de Salmonella

XerCD Recombinase de E. coli

XerCD Recombinase de E. coli

XerCD Recombinase de E. coli

Transposio

Transposio

Cut and Paste

Retrotransposons/

Retrovirus

Retrotransposons/Retrovirus

Recombinao/

Reparo e Evoluo

de Genomas

Mecanismos de duplicao gnica

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK21112/

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK21112/

Mecanismos de duplicao gnica

8.8 Morphological Complexity Evolves by Adding New Gene Functions

FIGURE 17: Common eukaryotic proteins are

concerned with essential cellular functions

FIGURE 18: Increasing complexity in eukaryotes is accompanied by

accumulation of new proteins for transmembrane and extracellular functions

Famlias de Genes/Protenas

8.10 Globin Clusters Arise by Duplication

and Divergence

FIGURE 20: Each of the alpha-like and beta-like globin gene families is organized

into a single cluster that includes functional genes and pseudogenes (psi)

8.10 Globin Clusters Arise by Duplication and Divergence

FIGURE 21: Different

hemoglobin genes are

expressed during

embryonic, fetal, and

adult periods of

human development

8.10 Globin Clusters Arise by Duplication

and Divergence

8.10 Globin Clusters Arise by Duplication and Divergence

FIGURE 22: Seven mouse genes include two early embryonic genes, one

late embryonic gene, two adult genes, and two pseudogenes

8.11 Pseudogenes Are Nonfunctional

Gene Copies

Some pseudogenes may gain functions different from

those of their parent genes, such as regulation of gene

expression, and take on different names.

FIGURE 23: Many

changes have

occurred in a beta-

globin gene since it

became a

pseudogene

FIGURE 24: Most human RP pseudogenes are of recent origin; many are

shared with the chimpanzee but absent from rodents

Adapted from S. Balasubramanian, et al., Genome Biol. 20 (2009): R2.

Genes Homeobox

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK21112/

Exon/Domain Shuffling

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK21112/

Exon Shuffling

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK21112/

Exon/Domain Shuffling

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK21112/

Brown, 2002. Genomes, 2nd ed.

http://www.ncbi.nlm.nih.gov/books/NBK2

1112/

Duplicao de genomas inteiros

Genoma de Plantas = Baguna!

http://chibba.agtec.uga.edu/duplication/

FIGURE 13: DNA content of the haploid genome increases with morphological

complexity of lower eukaryotes, but varies among higher eukaryotes

Genoma de Plantas = Baguna!

Hiptese 2R de duplicao do genoma de

vertebrados

Da mitocndria para o ncleo: NUMTs

Bhazkani-Covo et al., 2010. Molecular

Poltergeists: Mitochondrial DNA

Copies (numts) in Sequenced Nuclear

Genomes. PLoS Genetics, e:1000834.

Do cloroplasto para

o ncleo, eu at

entendo, mas do

cloroplasto de uma

alga para o ncleo

de um animal

Rumpho et al., 2011. J Exp Biol, 214:

3003-11.

Evoluo de Blocos de Genes:

Inverses Cromossmicas

Evoluo de Blocos de

Genes:

Inverses Cromossmicas

Evoluo de Blocos de Genes:

Inverses Cromossmicas

Ananina et al., 2004. GMB, 27:61-69.